Human Coenzyme Q10 (CoQ10) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人辅酶Q10（CoQ10）酶联免疫检测试剂盒（货号：CSB-E14081h），采用竞争法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中CoQ10的含量，检测灵敏度为3.12 ng/mL，线性范围为3.12-50 ng/mL。辅酶Q10是一种脂溶性醌类化合物，广泛分布于线粒体内膜，参与细胞能量代谢中的电子传递链并发挥抗氧化功能，其水平变化与心血管功能、神经退行性疾病及衰老过程密切相关。本试剂盒通过特异性抗体与样本中CoQ10结合，结合酶标反应显色，可实现科研场景下对生物样本中CoQ10含量的精准检测，支持能量代谢研究、疾病模型机制探索（如心肌缺血、帕金森病等）以及膳食补充剂效果评估等实验需求。操作流程包含样本预处理、孵育、洗涤和显色步骤，配备标准品和对照品，便于用户快速建立标准曲线并完成数据分析，为体外实验提供可靠工具。
别名：

N/A
缩写：

CoQ10
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 ng/mL-50 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	96
	Range %	91-101
1:40	Average %	99
	Range %	93-104
1:80	Average %	92
	Range %	87-96
1:160	Average %	90
	Range %	85-94

回收率：

The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	93	88-97