Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

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中文名称：

人几丁质酶3样蛋白2(CHI3L2)酶联免疫试剂盒
货号：

CSB-EL005347HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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人几丁质酶3样蛋白2(CHI3L2)酶联免疫试剂盒（CSB-EL005347HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CHI3L2含量。CHI3L2是一种糖基水解酶家族成员，缺乏酶活性，通过结合多糖和细胞表面受体调控炎症、组织重塑和肿瘤微环境。在关节炎、纤维化疾病和恶性肿瘤中高表达，是疾病进展的生物标志物和潜在治疗靶点。试剂盒检测范围为0.78 ng/ml-50 ng/ml，该产品可为炎症性疾病机制研究、纤维化模型评估或肿瘤微环境分析提供关键生物标志物检测支持，特别适用于基础医学、免疫学及分子病理学领域的体外样本检测，帮助研究者探索CHI3L2在疾病发生发展中的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CH3L2_HUMAN ELISA Kit; CHI3L2 ELISA Kit; Chitinase 3 like 2 ELISA Kit; Chitinase-3-like protein 2 ELISA Kit; Chondrocyte protein 39 ELISA Kit; OTTHUMP00000014028 ELISA Kit; YKL 39 ELISA Kit; YKL-39 ELISA Kit; YKL39 ELISA Kit
缩写：

CHI3L2
Uniprot No.：

Q15782
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/ml-50 ng/ml
灵敏度：

0.19 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Stem Cells
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CHI3L2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-91
1:2	Average %	101
	Range %	95-105
1:4	Average %	102
	Range %	98-109
1:8	Average %	96
	Range %	91-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.701

2.688

2.695

2.582

2.344

2.192

2.268

2.155

12.5

1.704

1.729

1.717

1.604

6.25

1.151

1.137

1.144

1.031

3.125

0.678

0.699

0.689

0.576

1.56

0.388

0.366

0.377

0.264

0.78

0.238

0.241

0.240

0.127

0.115

0.111

0.113

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Chitinase 3-like proteins as diagnostic and prognostic biomarkers of multiple sclerosis Hinsinger G et al,Mult Scler,2015

功能：

Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.
基因功能参考文献：
1. This study shown that CHI3L2 is regulated differently in healthy and late onset Alzheimer"s disease brain depending on the sex. PMID: 27653898
2. Structural analysis demonstrates that YKL-39 interacts with chitooligosaccharides through hydrogen bonds and hydrophobic interactions. Thermodynamic analysis indicates that binding of chitooligosaccharide to YKL-39 is mainly driven by enthalpy. PMID: 25477513
3. These data suggest that hYKL-39 is a novel growth and differentiation factor involved in cartilage homeostasis. PMID: 23291184
4. YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. PMID: 22742450
5. ERK1/2 phosphorylation by CHI3L2 inhibits cell mitogenesis and proliferation. PMID: 22211103
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亚细胞定位：

Secreted.
蛋白家族：

Glycosyl hydrolase 18 family
组织特异性：

Highest expression in chondrocytes, followed by synoviocytes, lung and heart. Not detected in spleen, pancreas, and liver. May also be expressed in developing brain and placenta.
数据库链接：

HGNC: 1933

OMIM: 601526

KEGG: hsa:1117

STRING: 9606.ENSP00000358763

UniGene: Hs.514840

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.