Human Centromere protein K(CENPK) ELISA kit

Human Centromere protein K(CENPK) ELISA kit

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中文名称：

人着丝粒蛋白K(CENPK)酶联免疫试剂盒
货号：

CSB-EL005214HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人着丝粒蛋白K(CENPK)酶联免疫试剂盒（CSB-EL005214HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CENPK含量。CENPK（着丝粒蛋白K）是一种与着丝粒复合体相关的亚基，其功能是将CENPA蛋白靶向到着丝粒，对于着丝粒功能和有丝分裂进程至关重要。研究显示，CENPK基因在肝细胞癌中异常高表达，其启动子区域的DNA低甲基化水平与其高表达密切相关，提示表观遗传学机制在肝细胞癌发生发展中起重要作用。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，可在科研场景中用于探索CENPK在细胞增殖、分化及癌症发生发展中的分子机制，尤其适用于染色体动力学研究、肿瘤模型构建以及药物干预对染色体稳定性影响的体外实验，适用于分子生物学、肿瘤学等领域的蛋白功能研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CENPK ELISA Kit; ICEN37 ELISA Kit; FKSG14 ELISA Kit; Centromere protein K ELISA Kit; CENP-K ELISA Kit; Interphase centromere complex protein 37 ELISA Kit; Protein AF-5alpha ELISA Kit; p33 ELISA Kit
缩写：

CENPK
Uniprot No.：

Q9BS16
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

23.5 pg/mL-1500 pg/mL
灵敏度：

5.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CENPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	100
	Range %	90-105
1:4	Average %	92
	Range %	85-96
1:8	Average %	92
	Range %	84-96

回收率：

The recovery of human CENPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-95
EDTA plasma (n=4)	99	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.889	1.966	1.928	1.798
750	1.426	1.476	1.451	1.321
375	0.921	0.900	0.911	0.781
187.5	0.566	0.543	0.555	0.425
94	0.369	0.345	0.357	0.227
47	0.259	0.263	0.261	0.131
23.5	0.199	0.192	0.196	0.066
0	0.131	0.129	0.130

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
相关疾病：

Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.
亚细胞定位：

Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
蛋白家族：

CENP-K/MCM22 family
组织特异性：

Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
数据库链接：

HGNC: 29479

OMIM: 611502

KEGG: hsa:64105

STRING: 9606.ENSP00000242872

UniGene: Hs.529778

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.