Human Cementum Protein 1 (CEMP-1) ELISA Kit

Human Cementum Protein 1 (CEMP-1) ELISA Kit

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中文名称：

人牙骨质蛋白1（CEMP-1）酶联免疫试剂盒
货号：

CSB-E13012h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专为定量检测人血清或血浆样本中的牙骨质蛋白1（CEMP-1）而设计。CEMP-1作为牙骨质特异性基质蛋白，在牙周组织再生、牙齿发育及骨代谢调控中发挥重要作用，是研究牙周疾病、骨骼矿化机制及口腔组织工程的关键生物标志物。试剂盒检测灵敏度达0.156 ng/mL，线性范围覆盖0.156-10 ng/mL，采用预包被96孔板形式，通过标准品梯度稀释建立定量曲线，实验全程包含阳性对照，确保检测数据的可靠性。该产品适用于体外实验研究，可用于探索牙周炎病理机制、评估牙骨质再生治疗方案的疗效，或作为骨代谢相关疾病的分子机制研究工具，为口腔医学、骨生物学等领域的科研工作者提供精准的蛋白定量解决方案。建议实验前通过预实验确定样本最佳稀释比例，避免基质效应对检测结果的影响。
别名：

Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit
缩写：

CEMP1
Uniprot No.：

Q6PRD7
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	101
	Range %	97-105
1:4	Average %	83
	Range %	80-86
1:8	Average %	107
	Range %	103-110

回收率：

The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	91	84-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.658	2.697	2.678	2.528
5	2.312	2.331	2.322	2.172
2.5	1.871	1.892	1.882	1.732
1.25	1.312	1.365	1.339	1.189
0.625	0.788	0.797	0.793	0.643
0.312	0.442	0.472	0.457	0.307
0.156	0.281	0.272	0.277	0.127
0	0.151	0.149	0.150

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Periodontal Specific Differentiation of Dental Follicle Stem Cells into Osteoblast, Fibroblast, and Cementoblast. Sowmya S.et al,Tissue Eng Part C Methods.,2015
Simulation study of natural UV-B radiation on Catla catla and its impact on physiology, oxidative stress, Hsp 70 and DNA fragmentation Moirangthem Kameshwor Singh. et al,Journal of Photochemistry and Photobiology B: Biology,2015

功能：

May play a role in development of the periodontium which surrounds and supports the teeth by promoting the differentiation of multi-potent cells from the periodontal ligament into cementoblasts to form the cementum. Binds hydroxyapatite and may promote the biomineralization of the cementum. Also promotes cell proliferation.
基因功能参考文献：
1. The synthesis of hydroxyapatite with different crystallinities by controlling the concentration of recombinant CEMP1 for biological application. PMID: 26652387
2. CEMP1 exerts modulation of a number of cellular genes. PMID: 26011628
3. when HIF-1 was activated by gene introduction or chemically, CEMP1 expression and mineralization increased. PMID: 24117017
4. human primary cementoblasts subjected to compression and IL-1beta stimulation impeded BSP and CEMP-1 expression, proteins that are associated with cementogenesis. PMID: 22349547
5. These data demonstrate for the first time that the CEMP1 is not only a marker protein for cementoblast-related cells, but it also regulates cementoblast commitment in periodontal ligament cells PMID: 21465469
6. CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and it might have a potential function in cementum and bone formation PMID: 17509525
7. CEMP1 plays a role during the biomineralization process by promoting octacalcium phosphate crystal nucleation. PMID: 19393626
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亚细胞定位：

Cytoplasm. Nucleus.
组织特异性：

Expressed by cementoblasts, a subpopulation of periodontal ligament cells and cells located around vessels in periodontium (at protein level).
数据库链接：

HGNC: 32553

OMIM: 611113

KEGG: hsa:752014

STRING: 9606.ENSP00000371787

UniGene: Hs.433499

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.