Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

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中文名称：

人抗菌肽(CAMP)酶联免疫试剂盒
货号：

CSB-EL004476HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人抗菌肽(CAMP)酶联免疫试剂盒（CSB-EL004476HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CAMP含量。CAMP（环腺苷酸）是细胞内重要的第二信使，参与多种生理过程。研究显示，失控的cAMP可能导致肝癌，其机制涉及cAMP在无膜细胞器中的动态隔离，当这些结构被破坏时，cAMP会淹没细胞，导致失控生长。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于体外科研实验中探索CAMP在感染性病原体应答、慢性炎症性疾病（如银屑病、炎症性肠病）或皮肤创伤愈合等生理病理过程中的表达变化，为免疫机制研究及潜在生物标志物分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37,
缩写：

CAMP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.45 ng/mL-30 ng/mL
灵敏度：

0.11 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
Range %	80-92
1:200	Average %	96
Range %	91-105
1:400	Average %	99
Range %	92-110
1:800	Average %	95
Range %	86-98

回收率：

The recovery of human CAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-98
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.713	2.664	2.689	2.612
15	1.687	1.567	1.627	1.550
7.5	1.047	0.989	1.018	0.941
3.75	0.563	0.543	0.553	0.476
1.8	0.339	0.324	0.332	0.255
0.9	0.206	0.196	0.201	0.124
0.45	0.145	0.139	0.142	0.065
0	0.080	0.074	0.077

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-α production in systemic sclerosis Roberto Lande,Nature Communications,2019
Double-stranded RNA induces cathelicidin expression in the intestinal epithelial cells through phosphatidylinositol 3-kinase-protein kinase Cζ-Sp1 pathway and ameliorates shigellosis in mice Atri Ta.et al,Cell Signal. ,2017
Anti-TNF therapy response in patients with ulcerative colitis is associated with colonic anti-microbial peptide expression and microbiota composition Magnusson M K, Strid H, Sapnara M, et al. ,J Crohns Colitis.,2016

■ 常见问题解答

we are interested in measurements of the Cathelicidin antimicrobial peptide (CAMP) in human fecal samples. According to your homepage, your assay is suitable for blood samples or tissue homogenates. But have you ever tested it in fecal samples? Would you expect it to work using fecal samples?

Thanks for your inquiry!
CSB-EL004476HU
We tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test human fecal samplee ,we suggest you do a pretest . Pls find following sample preparation method.
Feces Dilute samples with appropriate Sample Diluent. This can be achieved by adding 0.1g sample to appropriate Sample Diluent. Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.