Human Cartilage acidic protein 1(CRTAC1) ELISA kit

Human Cartilage acidic protein 1(CRTAC1) ELISA kit

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中文名称：

人软骨酸性蛋白1(CRTAC1)酶联免疫试剂盒
货号：

CSB-EL005997HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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人软骨酸性蛋白1（CRTAC1）是一种与软骨发育及功能密切相关的特异性蛋白，在软骨细胞外基质中参与细胞黏附与组织修复调控，其表达水平变化可能与骨关节炎、软骨损伤等病理过程相关。本试剂盒（货号CSB-EL005997HU）采用双抗体夹心法原理，可定量检测人血清、血浆及组织匀浆样本中的CRTAC1蛋白，检测灵敏度为4.68 ng/mL，线性范围为4.68-300 ng/mL，满足低浓度样本的精准分析需求。试剂盒包含预包被特异性抗体的酶标板、标准品、检测抗体及显色底物等核心组分，通过优化反应体系实现高重复性检测，操作流程涵盖样本孵育、洗涤、显色及终止步骤，可在常规实验条件下完成检测。该产品适用于软骨相关疾病机制研究、生物标志物筛选及药物干预效果评估等科研领域，尤其适合在血清、血浆等复杂基质中分析CRTAC1的动态变化，为软骨代谢研究提供可靠工具。
别名：

68 kDa chondrocyte expressed protein ELISA Kit; 68 kDa chondrocyte-expressed protein ELISA Kit; Acidic secreted protein in cartilage ELISA Kit; ASPIC ELISA Kit; ASPIC1 ELISA Kit; Cartilage acidic protein 1 ELISA Kit; Cartilage acidic protein 1 precursor ELISA Kit; CEP 68 ELISA Kit; CEP-68 ELISA Kit; CEP68 ELISA Kit; Chondrocyte expressed protein 68 kDa CEP 68 ELISA Kit; CRAC1_HUMAN ELISA Kit; CRTAC1 ELISA Kit; Protein CRTAC1-B ELISA Kit; W307 ELISA Kit
缩写：

CRTAC1
Uniprot No.：

Q9NQ79
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.68 ng/mL-300 ng/mL
灵敏度：

1.17 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CRTAC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	87-96
1:10	Average %	97
	Range %	93-101
1:20	Average %	102
	Range %	99-103
1:40	Average %	88
	Range %	86-91

回收率：

The recovery of human CRTAC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-93
EDTA plasma (n=4)	102	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.522	2.735	2.629	2.444
150	2.043	2.102	2.073	1.888
75	1.317	1.315	1.316	1.131
37.5	0.875	0.857	0.866	0.681
18.75	0.470	0.445	0.458	0.273
9.37	0.366	0.380	0.373	0.188
4.68	0.254	0.252	0.253	0.068
0	0.186	0.184	0.185

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Identification of Cardiometabolic Protein Biomarkers for Acute Myocardial Infarction Using Olink Proteomics X Tan, X Wang, S Xu, Y Zeng, G Zhang,Journal of Inflammation Research,2025

基因功能参考文献：
1. Confirm the increase of CRTAC1 in cartilage biopsies from OA patients undergoing joint replacement by real-time PCR and immunohistochemistry. Furthermore, we report that proinflammatory cytokines interleukin-1beta and tumor necrosis factor alpha upregulate CRTAC1 expression in primary articular chondrocytes and synovial fibroblasts. PMID: 27415616
2. It was shown for the first time the propensity of CRTAC1 to form amyloid-like structures, and it was hypothesized that the aggregating property of CRTAC1 may be related to its disease-association. PMID: 27862299
3. These results provided new insights into the mechanism of cataract development, and demonstrated that CRTAC1 could be a potentially novel target for cataract treatment. PMID: 27855397
4. CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage. PMID: 17074475
5. in bone fracture patients, 12 proteins were related to bone/cartilage metabolism, including: TGF-beta induced protein IG-H(3), cartilage acidic protein 1, procollagen C proteinase enhancer protein and TGF-beta receptor III. PMID: 17602227
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亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Expressed in the interterritorial matrix of articular deep zone cartilage (at protein level). Isoform 1 and isoform 2 are expressed in brain. Isoform 1 is detected in lung and chondrocytes. Detected in cartilage, bone, cultured chondrocytes and lung, and
数据库链接：

HGNC: 14882

OMIM: 606276

KEGG: hsa:55118

STRING: 9606.ENSP00000359629

UniGene: Hs.500736

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.