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  4. Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

说明书下载
  • 中文名称：
    人羧基肽酶N催化链(CPN1)酶联免疫试剂盒
  • 货号：
    CSB-EL005898HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人羧基肽酶N催化链(CPN1)酶联免疫试剂盒（CSB-EL005898HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPN1含量。CPN1是一种细胞分裂素/嘌呤核糖核苷核苷酶，参与植物生长促进激素细胞分裂素的代谢。研究发现，CPN1存在于细胞壁空间中，通过活化从根部运输的前体，影响水稻穗形成等生长过程，有望应用于提高作物产量。试剂盒检测范围为31.25 mU/mL-2000 mU/mL，适用于体外实验中探索CPN1在炎症反应调控、血管功能异常或代谢性疾病中的分子机制，也可用于评估不同实验条件下（如药物处理或基因干预）生物样本中CPN1的活性动态变化。该产品为研究CPN1在基础科研中的生物学功能及其作为潜在生物标志物的应用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ACBP ELISA Kit; Anaphylatoxin inactivator ELISA Kit; Arginine carboxypeptidase ELISA Kit; Carboxypeptidase N catalytic chain ELISA Kit; Carboxypeptidase N polypeptide 1 50 kD ELISA Kit; Carboxypeptidase N polypeptide 1 ELISA Kit; Carboxypeptidase N small subunit ELISA Kit; Carboxypeptidase N subunit 1 ELISA Kit; CBPN_HUMAN ELISA Kit; CPN ELISA Kit; CPN1 ELISA Kit; Kininase 1 ELISA Kit; Kininase-1 ELISA Kit; Kininase1 ELISA Kit; Lysine carboxypeptidase ELISA Kit; Plasma carboxypeptidase B ELISA Kit; SCPN ELISA Kit; Serum carboxypeptidase N ELISA Kit
  • 缩写：
    CPN1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 mU/mL-2000 mU/mL
  • 灵敏度：
    7.8 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CPN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 85-95    
    1:2 Average % 105    
    Range % 101-109    
    1:4 Average % 87    
    Range % 84-92    
    1:8 Average % 95    
    Range % 91-99    
  • 回收率：
    The recovery of human CPN1 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 93-103    
    EDTA plasma (n=4) 92 85-97    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    mU/ml OD1 OD2 Average Corrected    
    2000 1.998 2.112 2.055 1.916    
    1000 1.547 1.675 1.611 1.472    
    500 1.122 1.132 1.127 0.988    
    250 0.683 0.688 0.686 0.547    
    125 0.436 0.454 0.445 0.306    
    62.5 0.321 0.325 0.323 0.184    
    31.25 0.232 0.241 0.237 0.098    
    0 0.138 0.140 0.139 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

CPN1 antibodies

CPN1 Proteins

靶点详情

  • 功能：
    Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
  • 基因功能参考文献：
    1. CPN binds to fibrinogen and is present in a fibrin clot prepared from plasma. PMID: 23000409
    2. carboxypeptidase N regulates the biologic activity of SDF-1alpha by reducing the chemokine-specific activity PMID: 15718415
    3. This review summarizes the structure, enzymatic properties and function of this enzyme, including insights gained by the recent elucidation of the crystal structure of the CPN catalytic subunit and structural modeling of the non-catalytic 83 kDa subunit. PMID: 18039526
  • 相关疾病：
    Carboxypeptidase N deficiency (CPND)
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase M14 family
  • 组织特异性：
    Synthesized in the liver and secreted in plasma.
  • 数据库链接：

    HGNC: 2312

    OMIM: 212070

    KEGG: hsa:1369

    STRING: 9606.ENSP00000359446

    UniGene: Hs.2246

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