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  4. Human C-C motif chemokine 16(CCL16) ELISA kit

Human C-C motif chemokine 16(CCL16) ELISA kit

说明书下载
  • 中文名称：
    人CC趋化因子16(CCL16)酶联免疫试剂盒
  • 货号：
    CSB-EL004779HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO定量检测人CC趋化因子16（CCL16）的ELISA试剂盒（货号：CSB-EL004779HU），适用于血清、血浆及组织匀浆样本的检测。CCL16属于CC亚家族趋化因子，主要参与炎症反应、免疫细胞迁移及组织修复等过程，在肝脏疾病、肿瘤微环境调控及免疫相关疾病研究中具有重要价值。试剂盒采用双抗体夹心法原理，通过预包被高特异性捕获抗体和标记检测抗体实现靶蛋白定量分析，检测范围覆盖39.06 pg/mL至2500 pg/mL，可精准检测低丰度样本。该试剂盒适用于体外研究，支持科研人员探索CCL16在炎症性疾病机制、免疫细胞趋化行为调控、肝纤维化或肿瘤免疫微环境中的表达变化，为分子机制研究及生物标志物筛选提供可靠工具。实验操作标准化，兼容常规实验室设备，满足多种生物样本的定量分析需求。
  • 别名：
    C-C motif chemokine 16 ELISA Kit; CCL16 ELISA Kit; CCL16_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 16 ELISA Kit; Chemokine CC-4 ELISA Kit; Chemokine LEC ELISA Kit; CKb12 ELISA Kit; HCC-4 ELISA Kit; HCC4 ELISA Kit; IL-10-inducible chemokine ELISA Kit; IL10 inducible chemokine ELISA Kit; IL10 inducible chemokine ELISA Kit; ILINCK ELISA Kit; LCC-1 ELISA Kit; LCC1 ELISA Kit; Liver CC chemokine 1 precursor ELISA Kit; Liver expressed chemokine ELISA Kit; Liver-expressed chemokine ELISA Kit; LMC ELISA Kit; Lymphocyte and monocyte chemoattractant ELISA Kit; Monotactin 1 ELISA Kit; Monotactin-1 ELISA Kit; MTN-1 ELISA Kit; Mtn1 ELISA Kit; NCC-4 ELISA Kit; NCC4 ELISA Kit; New CC chemokine 4 ELISA Kit; SCYA16 ELISA Kit; SCYL4 ELISA Kit; Small inducible cytokine A16 precursor ELISA Kit; Small inducible cytokine A16 precursor ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 16 ELISA Kit; Small-inducible cytokine A16 ELISA Kit
  • 缩写：
    CCL16
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39.06 pg/mL-2500 pg/mL
  • 灵敏度：
    9.77 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    312.528

    312.182

    312.923

    313.120

    312.775

    312.676

    SD

    0.038

    0.038

    0.027

    0.042

    0.040

    0.045

    CV(%)

    5.574

    5.580

    3.956

    6.150

    5.863

    6.598

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CCL16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:2

    Average %

    90

    Range %

    80-95

    1:4

    Average %

    97

    Range %

    91-105

    1:8

    Average %

    99

    Range %

    92-110

    1:16

    Average %

    92

    Range %

    86-99

  • 回收率：

    The recovery of human CCL16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    89-98

    EDTA plasma (n=4)

    94

    91-100

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2500

    2.795

    2.880

    2.838

    2.680

    1250

    2.147

    2.142

    2.145

    1.987

    625

    1.317

    1.426

    1.372

    1.214

    312.5

    0.647

    0.667

    0.657

    0.499

    156.25

    0.367

    0.325

    0.346

    0.188

    78.13

    0.227

    0.244

    0.236

    0.078

    39.06

    0.174

    0.179

    0.177

    0.019

    0

    0.159

    0.157

    0.158

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

CCL16 antibodies

CCL16 Proteins

靶点详情

  • 最新研究进展：
        CCL16（也称为HCC-4）是一种由多种细胞产生的蛋白质，如巨噬细胞和淋巴细胞。它是一种趋化因子，可以吸引单核细胞、树突状细胞等炎症相关细胞的迁移。研究表明，CCL16参与了许多疾病的发生和发展，如肿瘤、类风湿性关节炎、自身免疫性疾病等。
     
  • 功能：
    Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
  • 基因功能参考文献：
    1. Overexpression of miR-1247 may alleviate LPS-induced A549 cell injury through targeting CCL16. PMID: 29768218
    2. Single nucleotide polymorphism (SNPs) located in and around the C-C motif chemokine ligand 16 (CCL16) gene are associated with CCL16 protein levels in blood plasma, and rs80329614 is the SNP most strongly associated with CCL16 protein levels in plasma. PMID: 27357396
    3. High umbilical artery CCL16 is prominently detected in preterm preeclamptic pregnancies with severe growth restriction. PMID: 22857868
    4. Data show that CCL-16 gene expression was upregulated by 7.46-fold in IBS patients when compared with controls and suggest a subclinical inflammatory component underlying IBS. PMID: 21951809
    5. Low umbilical cord blood chemokine CCL16 associates with spontaneous preterm birth PMID: 21288736
    6. Recombinant CCL16 significantly enhances the effector and the antigen-presenting function of macrophages and augments T cell lytic activity. PMID: 14525962
    7. An adenoviral vector encoding human CCL16 injected into mice with pre-established mammary carcinoma induces strong antitumor response; when given before surgical excision of primary lesions, the treatment prevents metastatic spread and cures 63% of mice. PMID: 15034014
    8. CCL16 induced efficient migratory responses in human and mouse eosinophils. CCL16 is a novel functional ligand for H4 and may have a role in trafficking of eosinophils. PMID: 15265943
    9. CC chemokine-4 (HCC-4)/CCL16 transduces signals differently from other CCR1-dependent chemokines and may play different roles in the immune response. PMID: 16226254
    10. Elevated concentrations in bronchoalveolar lavage fluid from patients with eosinophilic pneumonia PMID: 19494526

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
  • 数据库链接：

    HGNC: 10614

    OMIM: 601394

    KEGG: hsa:6360

    STRING: 9606.ENSP00000293275

    UniGene: Hs.10458

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |