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  4. Human B-cell differentiation antigen CD72(CD72) ELISA kit

Human B-cell differentiation antigen CD72(CD72) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    人B细胞分化蛋白CD72(CD72)酶联免疫试剂盒
  • 货号：
    CSB-EL004955HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法（ELISA）定量检测人血清、血浆及组织匀浆中的B细胞分化蛋白CD72，该蛋白属于C型凝集素家族膜蛋白，在B细胞发育和免疫调节中发挥重要作用，通过负向调控B细胞受体信号通路参与免疫耐受机制，其与CD5的相互作用在自身免疫性疾病研究中备受关注。本试剂盒检测灵敏度为0.9 ng/mL，线性范围覆盖0.9-60 ng/mL，包含预包被的捕获抗体、生物素标记的检测抗体及优化反应体系，实验全程约需3小时，标准品采用梯度冻干粉形式便于保存，试剂盒组分可短期4℃保存或长期-20℃冻存。适用于科研领域探究B细胞相关免疫机制，如系统性红斑狼疮、类风湿性关节炎等自身免疫疾病模型中的B细胞功能研究，或肿瘤微环境中B淋巴细胞活化状态的分子机制分析，为免疫调控网络研究提供定量检测方案。
  • 别名：
    CD72; B-cell differentiation antigen CD72; Lyb-2; CD antigen CD72
  • 缩写：
    CD72
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.9 ng/mL-60 ng/mL
  • 灵敏度：
    0.225 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CD72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 98
    Range % 92-103
    1:2 Average % 91
    Range % 86-98
    1:4 Average % 92
    Range % 88-97
    1:8 Average % 101
    Range % 96-15
  • 回收率：
    The recovery of human CD72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-95
    EDTA plasma (n=4) 101 103-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    60 2.588 2.787 2.688 2.535
    30 2.169 2.236 2.203 2.050
    15 1.674 1.744 1.709 1.556
    7.5 1.322 1.223 1.273 1.120
    3.75 0.752 0.744 0.748 0.595
    1.8 0.363 0.375 0.369 0.216
    0.9 0.261 0.255 0.258 0.105
    0 0.149 0.156 0.153 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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CD72 antibodies

CD72 Proteins

靶点详情

  • 功能：
    Plays a role in B-cell proliferation and differentiation.
  • 基因功能参考文献：
    1. Interferon-alpha-induced CD100 expression on naive CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction. PMID: 28222623
    2. Increased soluble CD72 in systemic lupus erythematosus is in association with disease activity and lupus nephritis PMID: 26883681
    3. CD72 expression on activated B cells of SLE patients was significantly lower than that of normal controls. sema3A enhanced CD72 expression of B cells, but it was still lower in SLE patients than in normal individuals. PMID: 24461079
    4. data demonstrated aberrant expression of CD72 exists on B cells of myasthenia gravis and multiple sclerosis patients and expression level of CD72 molecule has a significantly negative correlation with anti-AchR antibody levels in myasthenia gravis PMID: 23184497
    5. regulates serum immunoglobulin leveln and disease resistance in systemic lupus erythematosus PMID: 23268649
    6. CD72 mRNA expression level correlates with Sema4D expression in peripheral blood mononuclear cells in immune thrombocytopenia. PMID: 22111667
    7. CD100, CD72 and CD45 were expressed in placenta and exhibited different mRNA and protein levels in normal pregnancy and miscarriage. Protein levels were highly dysregulated around 10 weeks of gestation in first and second miscarriage placentas. PMID: 22606231
    8. CD100-CD72 interaction can be the mechanism by which NK cell communicate with B cells. PMID: 17786190
    9. Data show that ligation of CD72 with the BU40, or with rCD100 negatively regulates KIT-mediated mast cell proliferation, chemotaxis, and chemokine production. PMID: 20100931
    10. results indicated that the presence of CD72-*2 allele decreases risk for human systemic lupus erythematosus conferred by FCGR2B-232Thr, possibly by increasing the AS isoform of CD72 PMID: 15459183
    11. CD72 is a key molecule in regulating mature B cell differentiation; CD72 signaling reduces the expression of X-box binding protein 1 in B cells PMID: 16047337
    12. increased nucleotide mutation of CD72 mRNA accounts for the decreased expression level of CD72 in B cells, and it might be related to hyperactivity of B cells in patients with SLE PMID: 17121583
    13. CD72 polymorphisms are associated with age of appearing clinical manifestations in idiopathic thrombocytopenic purpura in Chinese patients. PMID: 18071878

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  • 亚细胞定位：
    Membrane; Single-pass type II membrane protein.
  • 组织特异性：
    Pre-B-cells and B-cells but not terminally differentiated plasma cells.
  • 数据库链接：

    HGNC: 1696

    OMIM: 107272

    KEGG: hsa:971

    STRING: 9606.ENSP00000259633

    UniGene: Hs.116481

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |