Human Azurocidin/Heparin Binding Protein(AZU/HBP) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO天青杀素/肝素结合蛋白（AZU/HBP）酶联免疫检测试剂盒，基于夹心法原理实现定量分析，适用于检测血清、尿液、组织匀浆及细胞培养上清液等多种样本中AZU/HBP的蛋白表达水平，检测灵敏度范围为7.8-500 pg/mL。AZU/HBP是一种由中性粒细胞分泌的阳离子抗菌蛋白，在先天免疫应答中发挥关键作用，其通过结合肝素和调控炎症反应参与宿主防御机制，并与感染性疾病的病理过程密切相关。该试剂盒采用特异性抗体对实现靶标捕获与检测，操作步骤标准化，可在4.5小时内完成实验流程，配套提供预包被酶标板、标准品、检测抗体及显色底物等完整组分，支持研究者定量分析AZU/HBP在炎症反应、免疫调控或感染模型中的动态变化，为探索其作为炎症相关生物标志物的功能机制提供可靠工具，广泛应用于免疫学、细胞生物学及疾病机制等科研领域。
缩写：

AZU1
Uniprot No.：

P20160
种属：

Homo sapiens (Human)
样本类型：

serum, urine, tissue homogenates, cell culture supernates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AZU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-91
1:2	Average %	95
	Range %	90-100
1:4	Average %	98
	Range %	92-107
1:8	Average %	92
	Range %	82-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
亚细胞定位：

Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

Peptidase S1 family, Elastase subfamily
数据库链接：

HGNC: 913

OMIM: 162815

KEGG: hsa:566

STRING: 9606.ENSP00000233997

UniGene: Hs.72885