Human Aquaporin 4 Antibody,AQP-4 Ab ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人水通道蛋白4抗体（AQP-4 Ab）酶联免疫检测试剂盒（货号：CSB-E13568h），采用双抗体夹心法定量检测血清或血浆样本中AQP-4抗体浓度，检测范围为1.56-100 ng/mL。水通道蛋白4（AQP-4）是中枢神经系统重要的跨膜水通道蛋白，参与脑脊液循环、血脑屏障功能及神经信号传导，其特异性抗体与视神经脊髓炎谱系疾病（NMOSD）等自身免疫性神经系统疾病密切相关。本试剂盒通过预包被高亲和力抗体与标记检测抗体的组合，实现对目标抗体的高灵敏度捕获与定量分析，适用于科研场景中疾病模型构建、抗体表达水平研究、药物干预效果评估等实验需求。实验样本支持血清和血浆类型，操作流程标准化，可在常规实验室条件下完成检测。该产品为探索AQP-4抗体在神经免疫疾病中的分子机制、筛选潜在治疗靶点等基础研究提供可靠工具，检测数据可服务于细胞实验、动物模型研究及生物标志物分析等方向。
缩写：

AQP-4 Ab
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-95
1:2	Average %	94
	Range %	84-105
1:4	Average %	97
	Range %	92-101
1:8	Average %	91
	Range %	82-97

回收率：

The recovery of human AQP-4 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-197
EDTA plasma (n=4)	95	84-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.