Human Apolipoprotein F(APOF) ELISA kit

中文名称：

人载脂蛋白F(APOF)酶联免疫试剂盒
货号：

CSB-EL001938HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人载脂蛋白F(APOF)酶联免疫试剂盒（CSB-EL001938HU）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOF含量。APOF（载脂蛋白F）是一种酸性唾液酸蛋白，存在于高密度和低密度脂蛋白上。研究表明，ApoF在动脉粥样硬化形成中可能发挥重要作用，其作用机制可能与血浆胆固醇水平不直接相关。近期研究显示，ApoF通过调节胆固醇代谢和胰岛β细胞功能，影响胰岛素分泌，从而在糖尿病等疾病中发挥作用。试剂盒检测范围为9.38 ng/mL-600 ng/mL，支持科研人员开展脂代谢机制、心血管疾病模型构建等基础研究，尤其适用于评估动物实验模型中肝脏、脂肪组织等部位的APOF表达水平，或探索其在脂质运输、炎症反应等分子通路中的功能；可满足细胞实验、疾病模型构建及相关生物标志物筛选等科研场景需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Apo-F ELISA Kit; Apof ELISA Kit; APOF_HUMAN ELISA Kit; Apolipoprotein F ELISA Kit; DKFZp781G18150 ELISA Kit; Lipid transfer inhibitor protein ELISA Kit; LTIP ELISA Kit; MGC22520 ELISA Kit
缩写：

APOF
Uniprot No.：

Q13790
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

9.38 ng/mL-600 ng/mL
灵敏度：

2.34 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APOF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	86-96
1:400	Average %	95
	Range %	91-99
1:800	Average %	88
	Range %	84-94
1:1600	Average %	87
	Range %	84-93

回收率：

The recovery of human APOF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
600	0.088	0.090	0.089
300	0.113	0.119	0.116
150	0.161	0.172	0.167
75	0.243	0.254	0.249
37.5	0.388	0.395	0.392
18.75	0.680	0.698	0.689
9.38	0.941	1.001	0.971
0	1.975	2.075	2.025

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
基因功能参考文献：
1. Direct interactions between C/EBPalpha and ETS-1 were important for high liver-specific expression of ApoF. PMID: 25726912
2. this study has identified apoF as likely another target gene of FXR. PMID: 24211198
3. LTIP translocation is dependent on LDL lipid composition PMID: 21937674
4. LTIP tailors CETP-mediated remodeling of HDL3 and HDL2 particles in subclass-specific ways, strongly implicating it as a regulator of HDL metabolism PMID: 12907677
5. we have confirmed the negative association between plasma triglyceride levels and Apolipoprotein F previously suggested by a small data set, but now we demonstrate that this effect is seen only in males. PMID: 17901467
6. lipoprotein composition may influence the status of lipid transfer inhibitor protein activity PMID: 18369235
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亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein F family
组织特异性：

Expressed by the liver and secreted in plasma.
数据库链接：

HGNC: 615

OMIM: 107760

KEGG: hsa:319

STRING: 9606.ENSP00000381250

UniGene: Hs.534302

Human Apolipoprotein F(APOF) ELISA kit

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