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  4. Human Adipsin ELISA Kit

Human Adipsin ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    人降脂素酶联免疫试剂盒
  • 货号：
    CSB-E14369h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人降脂素酶联免疫试剂盒（货号：CSB-E14369h）基于双抗体夹心原理开发，可定量检测血清、血浆及组织匀浆样本中0.312-20 ng/mL浓度范围内的人降脂素水平。该蛋白作为脂质代谢调控的关键因子，在脂肪细胞分化、胆固醇平衡及能量稳态中发挥重要作用，其异常表达与动脉粥样硬化、肥胖等代谢性疾病密切相关。试剂盒采用高特异性抗体对构建的夹心检测体系，灵敏度达0.078 ng/mL，可精准捕捉样本中微量目标蛋白，适用于基础医学研究中脂代谢调控机制探索、心血管疾病模型分析、以及降脂药物或营养干预的体外效果评估。实验全程仅需4-6小时，通过预包被96孔板实现标准化操作，配套提供标准品、检测抗体及显色系统，支持科研人员高效完成细胞实验、动物模型或临床前样本的定量分析。该试剂盒严格验证了样本基质适应性，组织匀浆样本需经离心预处理后检测，确保数据可靠性。
  • 别名：
    Adipsin ELISA Kit; Adipsin/complement factor D ELISA Kit; Adn ELISA Kit; C3 convertase activator ELISA Kit; CFAD_HUMAN ELISA Kit; CFD ELISA Kit; Complement factor D ELISA Kit; complement factor D preproprotein ELISA Kit; D component of complement ELISA Kit; DF ELISA Kit; FactorD ELISA Kit; PFD ELISA Kit; Properdin factor D ELISA Kit
  • 缩写：
    CFD
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    2.593

    2.559

    2.619

    2.671

    2.640

    2.602

    SD

    0.043

    0.036

    0.039

    0.057

    0.050

    0.048

    CV(%)

    5.089

    4.301

    4.583

    6.605

    5.841

    5.667

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human adipsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:500

    Average %

    87

    Range %

    82-91

    1:1000

    Average %

    84

    Range %

    80-88

    1:2000

    Average %

    104

    Range %

    98-108

    1:4000

    Average %

    90

    Range %

    85-94

  • 回收率：

    The recovery of human adipsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    94-103

    EDTA plasma (n=4)

    94

    90-100

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.591

    2.423

    2.507

    2.402

    10

    1.964

    1.890

    1.927

    1.822

    5

    1.336

    1.301

    1.319

    1.214

    2.5

    0.842

    0.836

    0.839

    0.734

    1.25

    0.449

    0.424

    0.437

    0.332

    0.63

    0.329

    0.339

    0.334

    0.229

    0.31

    0.249

    0.241

    0.245

    0.140

    0

    0.108

    0.102

    0.105

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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CFD antibodies

CFD Proteins

靶点详情

  • 最新研究进展：
    CFD（Complement factor D）是补体系统中的一种血清蛋白，主要参与剪切促进因子B（Factor B）的激活，从而启动替代途径的活化。最新研究表明，CFD在炎症、自身免疫疾病、感染和肿瘤等多种疾病中都起到了重要作用。例如，CFD可以促进炎症细胞的浸润和激活，导致组织损伤和炎症反应。此外，CFD也参与了肿瘤的生长、侵袭和转移等过程。
     
  • 功能：
    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • 基因功能参考文献：
    1. MASP-3 is the exclusive pro-factor D activator in resting blood: the lectin and the alternative complement pathways are fundamentally linked. PMID: 27535802
    2. disturbance of reciprocal relationships between adipsin and leptin in obesity is associated with the development of insulin resistance PMID: 28864895
    3. TZDs (pioglitazone, rosiglitazone, and ciglitazone) slow tumor cell growth in vitro and in vivo with decreases in cell proliferation and increases in PPARg and adipsin PMID: 28219679
    4. The strong association of PLIN1, CFD and ADIPOQ genes with adipogenesis prompted authors to study the influence the bone health status as evaluated by quantitative ultrasound (QUS) bone densitometer in a North Indian cohort. Overall, ADIPOQ (rs1501299 and rs3774261) and combined cluster of PLIN1 rs2304796 and rs2304795) and CFD (rs1683563) demonstrated correlation. PMID: 28189761
    5. our findings set up a novel mechanism for FABP4, adipsin and adiponectin through gut microbiota mediating expression in gut Paneth cells. PMID: 26687459
    6. Overall adipsin levels among male asbestos industry workers were significantly higher than among control subjects. PMID: 25840635
    7. Data indicate that a urinary protein, adipsin, was significantly increased in patients with preeclampsia. PMID: 24958499
    8. Study demonstrates that T2DM patients with beta cell failure are deficient in adipsin. PMID: 24995977
    9. Complement factor D (FD) is activated by its substrate through interactions outside the active site which lock the unbound native state into an ordered inactive conformation via the self-inhibitory loop in FD. PMID: 24598742
    10. We demonstrate novel secretion of adipsin and ASP by placental Hofbauer cells. PMID: 23956345
    11. Increased activation of the alternative complement pathway in vitreous was controlled by disease stage and genetic variation in the complement pathway, supporting a role for complement activation in macular degeneration disease pathogenesis. PMID: 22930722
    12. Anti factor D Fab fragment inhibits FD proteolytic function by interfering with macromolecular substrate access rather than by inhibiting FD catalysis. PMID: 22362762
    13. CFD regulates activation of the alternative complement pathway, which is implicated in age related macular degeneration pathogenesis. PMID: 22003108
    14. CFD may not play a major role in the genetic susceptibility to age-related macular degeneration. PMID: 21139680
    15. crystal structure of C3bB at 4 A and complex with factor D at 3.5 A; data show how factor B binding to C3b forms open "activation" state of C3bB; Factor D binds open conformation of factor B through a site distant from the catalytic center PMID: 21205667
    16. As adiponectin and adipsin levels in CSF did not correlate with their levels in plasma, it seems that there could be a secondary intrathecal synthesis of these adipocytokines in multiple sclerosis PMID: 19538214
    17. increased serum levels in patients with seasonal allergic rhinitis PMID: 19699327

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  • 相关疾病：
    Complement factor D deficiency (CFDD)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    HGNC: 2771

    OMIM: 134350

    KEGG: hsa:1675

    STRING: 9606.ENSP00000332139

    UniGene: Hs.155597

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |