Human Acylamino-acid-releasing enzyme(APEH) ELISA kit

中文名称：

人酰基氨基酸释放酶(APEH)酶联免疫试剂盒
货号：

CSB-EL001899HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人酰基氨基酸释放酶（APEH）定量检测试剂盒，采用双抗体夹心法原理设计，适用于血清、血浆、组织匀浆、细胞裂解液等样本的科研分析。APEH是一种广泛存在于真核生物中的丝氨酸水解酶，参与蛋白质代谢调控，其活性异常与神经退行性疾病、肿瘤发生等病理过程密切相关。该试剂盒检测灵敏度高，线性范围宽达31.25-2000 pg/mL，可精准捕捉样本中APEH的微量表达变化。实验操作采用预包被特异性捕获抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配合标准品建立的标准曲线完成定量分析。科研人员可通过本产品研究APEH在细胞代谢调控、疾病发生机制中的作用，或用于药物开发中靶点活性评估。试剂盒包含优化缓冲液、标准品及显色底物，提供详细的实验操作手册，确保实验重复性和数据可靠性，为体外蛋白水平研究提供高效工具。
别名：

AARE ELISA Kit; ACPH ELISA Kit; ACPH_HUMAN ELISA Kit; Acyl peptide hydrolase ELISA Kit; Acyl-peptide hydrolase ELISA Kit; Acylamino acid releasing enzyme ELISA Kit; Acylamino-acid-releasing enzyme ELISA Kit; Acylaminoacyl peptidase ELISA Kit; Acylaminoacyl-peptidase ELISA Kit; APEH ELISA Kit; APH ELISA Kit; N acylaminoacyl peptide hydrolase ELISA Kit; OPH ELISA Kit; Oxidized protein hydrolase ELISA Kit
缩写：

APEH
Uniprot No.：

P13798
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APEH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-87
1:2	Average %	98
	Range %	94-103
1:4	Average %	88
	Range %	84-92
1:8	Average %	108
	Range %	104-112

回收率：

The recovery of human APEH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	86-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.049	2.102	2.076	1.960
1000	1.604	1.586	1.595	1.479
500	1.080	1.102	1.091	0.975
250	0.651	0.672	0.662	0.546
125	0.424	0.402	0.413	0.297
62.5	0.262	0.271	0.267	0.151
31.25	0.206	0.199	0.203	0.087
0	0.117	0.115	0.116

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
基因功能参考文献：
1. Although APEH is primarily localized in the cytoplasm, a sub-fraction of this enzyme is sequestered at sites of nuclear damage following UVA irradiation or following oxidative stress. APEH interacts with the amino-terminal domain of XRCC1, and APEH facilitates both single-strand break repair and cell survival following exposure to H2O2. Thus, APEH as a novel proteolytic component of the DNA damage response. PMID: 28866241
2. APEH polymorphism has significant influence on valproic acid pharmacokinetics in Chinese population PMID: 27406852
3. Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
亚细胞定位：

Cytoplasm.
蛋白家族：

Peptidase S9C family
数据库链接：

HGNC: 586

OMIM: 102645

KEGG: hsa:327

STRING: 9606.ENSP00000296456

UniGene: Hs.517969

Human Acylamino-acid-releasing enzyme(APEH) ELISA kit

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