Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

产品详情

产品描述：

人酸性鞘磷脂样磷酸二酯酶3b(SMPDL3B)酶联免疫试剂盒（CSB-EL021850HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SMPDL3B含量。SMPDL3B是一种脂质调节磷酸二酯酶，主要在巨噬细胞和树突状细胞表面活性，影响巨噬细胞脂质组成和膜流动性。其通过破坏鞘脂膜代谢，促进脂肪性肝炎，并作为Toll样受体信号通路的负调节因子，在免疫应答中发挥作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品可为科研人员开展脂质代谢机制、肾脏病理研究、免疫调节相关信号通路探索提供可靠工具，尤其适用于体外模型中SMPDL3B蛋白表达量变化的定量分析，为疾病机理研究和药物开发建立基础实验数据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

SMPDL3B ELISA kit; ASML3B ELISA kit; ASMLPDAcid sphingomyelinase-like phosphodiesterase 3b ELISA kit; ASM-like phosphodiesterase 3b ELISA kit; EC 3.1.4.- ELISA kit
缩写：

SMPDL3B
Uniprot No.：

Q92485
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SMPDL3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-89
1:2	Average %	92
	Range %	89-97
1:4	Average %	100
	Range %	95-105
1:8	Average %	90
	Range %	87-93

回收率：

The recovery of human SMPDL3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	99	95-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).
基因功能参考文献：
1. Glomerular immunoreactivity and urinary excretion of SMPDL3b in proteinuric nephrotic syndrome patients decreased compared with controls. PMID: 29081073
2. These results demonstrate that radiation injury induces early cytoskeletal remodeling, down-regulation of SMPDL3b, and elevation of cellular ceramide levels. Overexpression of SMPDL3b and pretreatment with rituximab confer a radioprotective effect in cultured podocytes. PMID: 27836988
亚细胞定位：

Secreted. Cell membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

Acid sphingomyelinase family
数据库链接：

HGNC: 21416

OMIM: 617737

KEGG: hsa:27293

STRING: 9606.ENSP00000363001

UniGene: Hs.123659