Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

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中文名称：

人ATP合成酶脂质结合蛋白，线粒体(ATP5G1)酶联免疫试剂盒
货号：

CSB-EL002359HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人ATP合成酶脂质结合蛋白（ATP5G1）定量检测试剂盒，采用双抗体夹心法原理设计，可精准检测血清、血浆、组织匀浆及细胞裂解液等多种样本中ATP5G1的浓度水平，检测灵敏度范围为23.5-1500 pg/mL。ATP5G1是线粒体ATP合酶复合体的重要亚基，通过调控质子跨膜转运参与细胞能量代谢的核心过程，其表达水平与线粒体功能及氧化磷酸化效率密切相关。该试剂盒适用于基础科研领域对能量代谢机制的研究，可用于探索细胞代谢异常、神经退行性疾病模型中线粒体功能障碍、心血管疾病能量代谢调控，以及衰老相关线粒体活性变化等课题。实验操作采用预包被特异性捕获抗体与标记检测抗体协同作用，通过标准曲线实现目标蛋白的定量分析，具备良好的重复性与特异性，为体外实验提供可靠的检测方案。
别名：

ATP5MC1; ATP5G1; ATP synthase F(0 complex subunit C1, mitochondrial; ATP synthase lipid-binding protein; ATP synthase membrane subunit c locus 1; ATP synthase proteolipid P1; ATP synthase proton-transporting mitochondrial F(0 complex subunit C1; ATPase protein 9; ATPase subunit c
缩写：

ATP5G1
Uniprot No.：

P05496
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

23.5 pg/mL-1500 pg/mL
灵敏度：

5.86 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5G1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-92
1:2	Average %	94
	Range %	89-98
1:4	Average %	89
	Range %	85-93
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of human ATP5G1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.637	2.612	2.625	2.480
750	2.215	2.118	2.167	2.022
375	1.697	1.683	1.690	1.545
187.5	1.107	1.118	1.113	0.968
94	0.611	0.621	0.616	0.471
47	0.365	0.372	0.369	0.224
23.5	0.242	0.231	0.237	0.092
0	0.146	0.144	0.145

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Fo ATP synthase C subunit serum levels in patients with ST-segment Elevation Myocardial Infarction: Preliminary findings Campo G.et al,Int J Cardiol.,2016

功能：

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
基因功能参考文献：
1. ATP5G1, ATP5G2, and ATP5G3 of the ATP synthase are not involved in forming the permeability transition pore. PMID: 28289229
2. ATP5G1 turnover increases upon depletion of ZC3H14, double knockdown of ZC3H14 and the nonsense-mediated decay factor, UPF1, rescues ATP5G1 transcript levels. Furthermore, fractionation reveals an increase in the amount of ATP5G1 pre-mRNA that reaches the cytoplasm when ZC3H14 is depleted and that ZC3H14 binds to ATP5G1 pre-mRNA in the nucleus. PMID: 27563065
3. The subunit c isoforms are nonredundant, because they differ functionally by their targeting peptides, which, in addition to mediating mitochondrial protein import, play a yet undiscovered role in respiratory chain maintenance. PMID: 19889836
亚细胞定位：

Mitochondrion membrane; Multi-pass membrane protein.
蛋白家族：

ATPase C chain family
数据库链接：

HGNC: 841

OMIM: 603192

KEGG: hsa:516

STRING: 9606.ENSP00000348205

UniGene: Hs.80986

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.