Human A disintegrin and metalloproteinase with thrombospondin motifs 2(ADAMTS2) ELISA kit

中文名称：

人金属肽酶含血小板反应蛋白2(ADAMTS2)酶联免疫试剂盒
货号：

CSB-EL001308HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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人金属肽酶含血小板反应蛋白2(ADAMTS2)酶联免疫试剂盒（CSB-EL001308HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTS2含量。ADAMTS2是一种细胞外金属蛋白酶，参与细胞外基质破坏、血管平滑肌细胞分型与凋亡、炎症细胞浸润、氧化应激等复杂机制。其在心脏肥厚、肝纤维化等疾病中发挥重要作用，通过调节PI3K/Akt信号通路影响心肌细胞肥大反应。试剂盒检测范围为3.12 ng/mL-200 ng/mL，支持科研人员探究 ADAMTS2 在皮肤弹性维持、器官纤维化病理机制或肿瘤微环境调控中的作用，为疾病模型构建、药物靶点筛选及生物标志物研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

A disintegrin and metalloproteinase with thrombospondin motifs 2 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 2 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 2 ELISA Kit; ADAM TS 2 ELISA Kit; ADAM TS2 ELISA Kit; ADAM-TS 2 ELISA Kit; ADAM-TS2 ELISA Kit; ADAMTS 3 ELISA Kit; ADAMTS-2 ELISA Kit; ADAMTS2 ELISA Kit; ATS2_HUMAN ELISA Kit; EC 3.4.24.14 ELISA Kit; EDS VIIB ELISA Kit; EDS VIIC ELISA Kit; hPCPNI ELISA Kit; NPI ELISA Kit; PC I NP ELISA Kit; PC I-NP ELISA Kit; PCINP ELISA Kit; PCPNI ELISA Kit; pNPI ELISA Kit; Procollagen I N proteinase ELISA Kit; Procollagen I N-proteinase ELISA Kit; Procollagen I/II amino propeptide processing enzyme ELISA Kit; Procollagen I/II amino propeptide-processing enzyme ELISA Kit; Procollagen N endopeptidase ELISA Kit; Procollagen N-endopeptidase ELISA Kit
缩写：

ADAMTS2
Uniprot No.：

O95450
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	92
	Range %	88-96
1:4	Average %	87
	Range %	82-91
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of human ADAMTS2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-109
EDTA plasma (n=4)	97	92-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.129	2.238	2.184	2.023
100	1.737	1.682	1.710	1.549
50	1.112	1.174	1.143	0.982
25	0.719	0.741	0.730	0.569
12.5	0.480	0.459	0.470	0.309
6.25	0.360	0.350	0.355	0.194
3.12	0.239	0.247	0.243	0.082
0	0.159	0.163	0.161

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form with reduced collagen-binding activity.
基因功能参考文献：
1. We identified three novel homozygous loss-of-function mutations (c.2927_2928delCT, p.(Pro976Argfs*42); c.669_670dupG, p.(Pro224Argfs*24); and c.2751-2A>T) and one compound heterozygous mutation (c.2T>C, p.? and c.884_887delTGAA, p.(Met295Thrfs26*)) in ADAMTS2 in five patients from four unrelated families PMID: 26765342
2. Data indicate that ADAMTS2 and 3 cleave the amino-propeptide of fibrillar collagens and regulate blood vessels homeostasis and lymphangiogenesis. Also, ADAMTS2 deficiency leads to the dermatosparactic type of Ehlers-Danlos syndrome. [review] PMID: 25863161
3. IL-1a is a strong positive regulator of ADAMTS-2 and ADAMTS-3 expression. PMID: 26232334
4. ADAMTS2 was significantly overexpressed in Fibrous dysplasia (FD) tissues, but rarely expressed in normal bone tissues, suggesting that ADAMTS2 could be a potential biomarker for FD PMID: 25674217
5. Pathway inhibition studies revealed that ADAMTS-2 upregulation by IL-6 was mediated by JNK pathway. PMID: 24752352
6. Data show that ADAMTS-2 is able to reduce proliferation of endothelial cells, and to induce their retraction and detachment from the substrate resulting in apoptosis. PMID: 20574651
7. Determination of the processing, activity and cleavage specificity of the bovine ADAMTS2 protein. PMID: 16046392
8. when conditioned media of TSP2-transfectants were added to cultures of bovine pulmonary microvascular endothelial cells (BPMEC), the BPMEC proliferation was significantly inhibited; suggesting that human TSP2 is a potential inhibitor of angiogenesis PMID: 11788898
9. Although exons 3-5 or 14-16 encode protein domains that have not been recognized as crucial for ADAMTS-2 activity, the aminoprocollagen processing was strongly impaired, providing evidence for the requirement of these domains for proper enzyme function PMID: 15373769
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相关疾病：

Ehlers-Danlos syndrome 7C (EDS7C)
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain.
数据库链接：

HGNC: 218

OMIM: 225410

KEGG: hsa:9509

STRING: 9606.ENSP00000251582

UniGene: Hs.23871

Human A disintegrin and metalloproteinase with thrombospondin motifs 2(ADAMTS2) ELISA kit

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