Human 8-iso prostaglandin F2α,8-iso-PGF2a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人8异前列腺素F2α（8-iso-PGF2α）酶联免疫试剂盒（货号：CSB-E12100h），专为定量检测血清、血浆样本中8-iso-PGF2α水平而设计。8-iso-PGF2α是脂质过氧化反应的稳定代谢产物，作为氧化应激的生物标志物，广泛参与炎症反应、心血管疾病及代谢紊乱等病理过程的研究。试剂盒基于竞争法原理，通过特异性抗体与样本中的目标分子结合实现检测，其灵敏度覆盖125 pg/mL至5000 pg/mL的线性范围，能够满足不同浓度样本的精准分析需求。该试剂盒操作流程简便，仅需常规实验室设备即可完成，样本无需复杂前处理，适用于细胞氧化损伤机制研究、抗氧化药物筛选评价以及环境或营养因素对机体氧化应激影响的科学探索。通过标准化实验方案和高品质预包被板设计，确保实验结果的重复性和可靠性，为氧化应激相关领域的基础研究与转化医学提供高效检测工具。
别名：

8-iso prostaglandin F2α,8-iso prostaglandin F2 alpha,8-iso-PGF2α,8-iso-PGF2 alpha
缩写：

8-iso-PGF2a
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

125 pg/mL-5000 pg/mL
灵敏度：

125 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 8-iso-PGF2a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	92
	Range %	85-99
1:4	Average %	95
	Range %	90-103
1:8	Average %	94
	Range %	90-99

回收率：

The recovery of human 8-iso-PGF2a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-102
EDTA plasma (n=4)	97	91-103