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Human 8-epi-prostaglandin F2alpha,8-epi-PGF2α ELISA Kit

  • 中文名称:
    人8-异构前列腺素F2α(8-epi-PGF2α)酶联免疫试剂盒
  • 货号:
    CSB-E09441h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    人8-异构前列腺素F2α(8-epi-PGF2α)酶联免疫试剂盒(CSB-E09441h)为双抗夹心法ELISA试剂盒,定量检测血清、尿液、组织匀浆样本中的8-epi-PGF2α含量。试剂盒检测范围为78 pg/mL-5000 pg/mL,该产品适用于体外研究样本中氧化应激水平的动态监测,可应用于动物模型或细胞实验中评估抗氧化治疗效果、疾病进程中氧化损伤程度等科研场景,为探索氧化应激相关分子机制提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    8-epi-prostaglandin F2α,8-epi-PGF2α,8-epi-PGF2 alpha
  • 缩写:
    8-epi-PGF2α
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, urine, tissue homogenates
  • 检测范围:
    78 pg/mL-5000 pg/mL
  • 灵敏度:
    19.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human 8-epi-PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %87-103
    1:2Average %86
    Range %82-93
    1:4Average %100
    Range %95-103
    1:8Average %93
    Range %84-100
  • 回收率:
    The recovery of human 8-epi-PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-103
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.965 2.897 2.931 2.820
    25002.211 2.323 2.267 2.156
    12501.599 1.566 1.583 1.472
    6250.811 0.802 0.807 0.696
    3120.505 0.474 0.490 0.379
    1560.311 0.301 0.306 0.195
    780.251 0.234 0.243 0.132
    00.113 0.109 0.111  
  • 数据处理:
  • 货期:
    3-5 working days

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