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中文名称:人8-异构前列腺素F2α(8-epi-PGF2α)酶联免疫试剂盒
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货号:CSB-E09441h
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:人8-异构前列腺素F2α(8-epi-PGF2α)酶联免疫试剂盒(CSB-E09441h)为双抗夹心法ELISA试剂盒,定量检测血清、尿液、组织匀浆样本中的8-epi-PGF2α含量。试剂盒检测范围为78 pg/mL-5000 pg/mL,该产品适用于体外研究样本中氧化应激水平的动态监测,可应用于动物模型或细胞实验中评估抗氧化治疗效果、疾病进程中氧化损伤程度等科研场景,为探索氧化应激相关分子机制提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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别名:8-epi-prostaglandin F2α,8-epi-PGF2α,8-epi-PGF2 alpha
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缩写:8-epi-PGF2α
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种属:Homo sapiens (Human)
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样本类型:serum, urine, tissue homogenates
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检测范围:78 pg/mL-5000 pg/mL
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灵敏度:19.5 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Others
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human 8-epi-PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 87-103 1:2 Average % 86 Range % 82-93 1:4 Average % 100 Range % 95-103 1:8 Average % 93 Range % 84-100 -
回收率:
The recovery of human 8-epi-PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-103 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 5000 2.965 2.897 2.931 2.820 2500 2.211 2.323 2.267 2.156 1250 1.599 1.566 1.583 1.472 625 0.811 0.802 0.807 0.696 312 0.505 0.474 0.490 0.379 156 0.311 0.301 0.306 0.195 78 0.251 0.234 0.243 0.132 0 0.113 0.109 0.111 -
数据处理:
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货期:3-5 working days
引用文献
- Association between short-term exposure to PM2. 5 and nasal microbiota dysbiosis, inflammation and oxidative stress: A panel study of healthy young adults W Li,Ecotoxicology and environmental safety,2023
- Bradykinin Protects Human Endothelial Progenitor Cells from High-Glucose-Induced Senescence through B2 Receptor-Mediated Activation of the Akt/eNOS Signalling Pathway Yuehuan Wu,Journal of Diabetes Research,2021
- The Value of 8-iso Prostaglandin F2 Alpha and Superoxide Dismutase Activity as a Clinical Indicator of Oxidative Stress in Type II Diabetes Mellitus MORSI, et al,Journal of Clinical & Diagnostic Research,2018
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