Human 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人6酮前列腺素F1a(6-keto-PGF1a)酶联免疫试剂盒（CSB-E09420h）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的6ketoPGF1a含量。人6酮前列腺素F1a（6-keto-PGF1a）是一种由前列腺素I2（PGI2）代谢生成的稳定产物，其在人体内具有调节血管功能和血小板聚集的作用。6-keto-PGF1a的研究背景主要涉及其在心血管疾病、炎症反应和肿瘤发生发展中的作用机制。试剂盒检测范围为50 pg/mL-800 pg/mL，适用于心血管疾病机制研究、药物开发中前列环素代谢评估以及内皮功能相关实验，可为血管生物学、药理学及病理生理学研究提供高效分析工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

6ketoPGF1a
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

50 pg/mL-800 pg/mL
灵敏度：

50 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 6-K-PGF1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-99
1:2	Average %	97
	Range %	93-105
1:4	Average %	85
	Range %	82-89
1:8	Average %	90
	Range %	85-98

回收率：

The recovery of human 6-K-PGF1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	95	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.