Human 6-hydroxymelatonin sulfate (6HMS) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测人血清、血浆及组织匀浆中6-羟基硫酸褪黑素（6HMS）的酶联免疫试剂盒，采用竞争法原理设计，通过样本中天然6HMS与酶标记物竞争结合预包被抗体，经显色反应后测定吸光度值实现精准定量。6HMS是褪黑素在肝脏代谢的主要硫酸化产物，其水平变化与褪黑素代谢调控密切相关，在昼夜节律研究、氧化应激评估及内分泌调节机制探索中具有重要科研价值。试剂盒检测灵敏度达10 pg/mL，线性范围为10-400 pg/mL，适用于检测低浓度生物样本，标准曲线显示板内重复性良好。实验流程包含样本预处理、孵育竞争结合、洗涤显色及终止反应等步骤，全程耗时约3小时，配套提供预包被96孔板、标准品、检测抗体、显色底物及终止液等完整组件。该试剂盒可应用于褪黑素代谢通路研究、神经内分泌系统功能分析，或探索6HMS在睡眠障碍、神经退行性疾病及免疫调节中的潜在作用机制，为体外实验提供稳定可靠的检测工具。
缩写：

6HMS
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 pg/mL- 400 pg/mL
灵敏度：

10 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 6HMS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-98
1:2	Average %	95
	Range %	87-105
1:4	Average %	96
	Range %	89-102
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of human 6HMS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.