Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗夹心酶联免疫吸附法（ELISA）检测人血清、血浆样本中的5-羟二十碳四烯酸（5-HETE）含量，其检测灵敏度为31.25 pg/mL，线性检测范围为31.25-2000 pg/mL，可在4-6小时内完成检测流程。5-HETE作为花生四烯酸通过脂氧合酶途径代谢的关键产物，在炎症反应、细胞增殖调控及免疫调节中发挥重要作用，其水平变化与多种病理生理过程相关。该试剂盒包含预包被特异性抗体的96孔板、标准品、生物素标记检测抗体、链霉亲和素-HRP及显色底物，实验需自备酶标仪（需具备450nm波长检测功能）、移液器和恒温培养箱等基础设备。本产品适用于体外定量分析生物样本中5-HETE的表达水平，为研究炎症相关疾病机制、评估药物干预效果或探索代谢通路调控提供可靠的实验工具，广泛应用于生命科学基础研究、药理学研究及生物标志物开发等领域。
缩写：

5-HETE
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HETE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
Range %	84-94
1:200	Average %	92
Range %	87-99
1:400	Average %	93
Range %	85-97
1:800	Average %	97
Range %	90-104

回收率：

The recovery of human 5-HETE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	91	84-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.