Human 3-nitrotyrosine (3-NT) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本试剂盒（CSB-E14324h）采用夹心法酶联免疫原理，专为定量检测人血清、血浆及组织匀浆样本中的3-硝基酪氨酸（3-Nitrotyrosine，3-NT）设计。3-硝基酪氨酸是蛋白质酪氨酸残基在活性氮物质作用下发生硝化修饰的标志性产物，其水平升高与氧化应激反应密切相关，常用于研究心血管疾病、神经退行性疾病及糖尿病并发症等病理过程中自由基损伤机制。试剂盒检测灵敏度高，线性范围覆盖0.156至10 ng/mL，可精准识别低丰度目标物。操作流程包括样本预处理（需离心去除沉淀物以避免堵塞）、标准品与样本同步孵育、生物素标记抗体结合、辣根过氧化物酶（HRP）催化显色及吸光度测定。适用于体外研究领域，如氧化应激相关疾病模型的分子机制探索、抗氧化药物筛选及药效评估等实验场景，配套提供预包被酶标板、标准品、检测抗体及终止液等完整组分，实验重复性良好，批次间稳定性严格质控，满足科研场景下对样本中3-NT含量的精准定量需求。
缩写：

3-NT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 3-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	90
	Range %	82-98
1:4	Average %	104
	Range %	99-111
1:8	Average %	86
	Range %	82-96

回收率：

The recovery of human 3-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	91	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.