Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测人血清、血浆、组织匀浆及细胞裂解液中2,3-二磷酸甘油酸（2,3-DPG）含量的酶联免疫试剂盒，采用双抗体夹心法原理进行精准检测。2,3-DPG是红细胞糖酵解的重要中间产物，通过调控血红蛋白与氧气的亲和力，在组织氧释放和缺氧适应中发挥关键作用。试剂盒检测范围为0.312-20 μmol/mL，可灵敏识别样本中微量2,3-DPG的存在，适用于基础研究中红细胞代谢功能评估、缺氧生理机制探索以及血液系统疾病模型的机制分析，例如贫血、高原反应或心血管相关疾病的体外实验研究。试剂盒提供预包被抗体板及标准品，操作流程标准化，仅需100 μL样本即可完成检测，配套试剂经过严格验证，具备良好的批间一致性和稳定性，满足细胞能量代谢、运动生理学或病理状态下的氧运输调控等科研需求。建议使用新鲜样本进行检测，避免反复冻融影响检测精度。
缩写：

2,3DPG
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.312 μmol/mL-20 μmol/mL
灵敏度：

0.078 μmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	92-100
1:2	Average %	98
Range %	92-103
1:4	Average %	95
Range %	90-99
1:8	Average %	90
Range %	85-96

回收率：

The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	84	80-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.