Horse insulin (INS) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO马胰岛素（INS）酶联免疫试剂盒（货号：CSB-EL011742HO），基于双抗体夹心酶联免疫吸附法（ELISA）原理开发，适用于定量检测马源血清、血浆及组织匀浆样本中的胰岛素水平。胰岛素作为关键的代谢调节激素，在维持血糖稳态和促进细胞葡萄糖摄取中发挥重要作用，其异常表达与多种代谢性疾病密切相关。本试剂盒检测范围为1.56-100 nIU/mL，最低检测限为1.56 nIU/mL，采用高特异性抗体确保检测的准确性和灵敏度。样本需预先离心去除杂质，避免反复冻融或细菌污染以保证检测稳定性。该产品可应用于动物科研领域，例如研究马的糖代谢调控机制、胰岛素相关疾病模型构建、营养干预或药物作用下的胰岛素分泌变化分析，以及运动生理学中能量代谢的动态监测等方向，为探索胰岛素在马体内的生物学功能提供可靠工具。
别名：

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
缩写：

INS
Uniprot No.：

P01310
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 nIU/mL-100 nIU/mL
灵敏度：

0.39 nIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	96-102
1:2000	Average %	87
	Range %	84-91
1:4000	Average %	95
	Range %	90-99
1:8000	Average %	98
	Range %	94-102

回收率：

The recovery of horse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	104	100-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
亚细胞定位：

Secreted.
蛋白家族：

Insulin family