Horse hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) ELISA kit

产品详情

产品描述：

马缺氧诱导因子1，α亚基(HIF1A)酶联免疫试剂盒（CSB-EL010351HO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HIF1A含量。HIF1A是一个重要靶点。它是低氧诱导因子1的α亚基，在细胞对低氧环境应答中起关键作用。研究发现其参与肿瘤生长、转移和血管生成等过程。针对它的研究机制多聚焦于调控其表达及活性，以寻找抗癌等疾病的新疗法。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，如通过检测动物模型血清/组织中的HIF1A水平，评估缺氧相关信号通路活性或药物对缺氧适应性的调控作用，还能为心血管疾病、肿瘤生物学等领域的体外实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

HIF1A
Uniprot No.：

F6VG41
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse HIF1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	102
	Range %	96-107
1:4	Average %	92
	Range %	87-98
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of horse HIF1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	103	92-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
2000	1.899	1.957	1.928	1.819
1000	1.417	1.496	1.457	1.348
500	0.926	0.978	0.952	0.843
250	0.583	0.572	0.578	0.469
125	0.345	0.353	0.349	0.240
62.5	0.246	0.252	0.249	0.140
31.25	0.171	0.178	0.175	0.066
0	0.108	0.109	0.109