Horse growth hormone,GH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO马生长激素（GH）酶联免疫检测试剂盒（货号：CSB-E11242Eq），基于竞争法定量检测马血清、血浆及组织匀浆样本中的GH水平。GH是垂体前叶分泌的多肽类激素，在动物生长发育、代谢调节及免疫应答中起关键作用。CUSABIO试剂盒采用高特异性抗体，检测灵敏度覆盖25 ng/mL至400 ng/mL的线性范围，适用于科研领域探究马匹生长调控、营养代谢、繁殖性能及疾病模型中的GH动态变化。实验流程包含预包被板、样本孵育、酶标抗体结合及显色反应，操作全程可在3.5小时内完成，配套提供标准品、稀释液及终止液等组分。试剂盒支持批量样本检测，适用于实验室对马科动物生理病理机制研究、激素相关药物开发评估以及畜牧领域GH水平监测等科研需求，检测结果稳定可靠，重复性良好，为科研工作者提供高效精准的GH定量分析工具。
别名：

GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：

GH1
Uniprot No.：

P01245
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

25 ng/mL-400 ng/mL
灵敏度：

25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	87
	Range %	84-90
1:4	Average %	96
	Range %	90-102
1:8	Average %	90
	Range %	85-96

回收率：

The recovery of horse GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-98
EDTA plasma (n=4)	96	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能参考文献：
1. Plasma levels of growth hormone (as well as glucose, insulin, and leptin) are highly correlated with the duration of winter anovulatory phase. PMID: 21958120
亚细胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
数据库链接：

KEGG: ecb:100034180

STRING: 9796.ENSECAP00000007142

UniGene: Eca.12604