Horse Tumor necrosis factor α,TNF-α ELISA Kit

Horse Tumor necrosis factor α,TNF-α ELISA Kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

马肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
货号：

CSB-E14031Hs
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品描述：

CUSABIO马肿瘤坏死因子α（TNF-α）酶联免疫检测试剂盒（货号：CSB-E14031Hs），采用双抗体夹心法定量分析血清、血浆及组织匀浆样本中TNF-α的含量，检测灵敏度为15.6 pg/mL，线性范围为15.6-1000 pg/mL，满足多种实验需求。TNF-α是一种重要的促炎细胞因子，参与调节免疫应答、炎症反应及细胞凋亡过程，其表达水平变化与感染性疾病、自身免疫异常及组织损伤修复等生理病理机制密切相关。本试剂盒通过高特异性抗体对目标蛋白进行捕获与检测，适用于科研场景下对马属动物炎症相关模型的机制研究、药物干预效果评价或基础免疫学分析，例如通过检测血清或组织中的TNF-α浓度动态，揭示炎症相关疾病的分子调控网络。该试剂盒操作简便，支持多种生物样本类型，实验过程中无需复杂前处理，配套试剂及标准品均经过严格验证，可为实验数据的准确性和重复性提供保障。注：本产品仅限科学研究使用。
别名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写：

TNF
Uniprot No.：

P29553
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-97
1:2	Average %	92
	Range %	84-99
1:4	Average %	95
	Range %	92-103
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of horse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	83-98
EDTA plasma (n=4)	94	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.601	2.548	2.575	2.409
500	2.248	2.211	2.230	2.064
250	1.631	1.616	1.624	1.458
125	0.905	0.925	0.915	0.749
62.5	0.475	0.483	0.479	0.313
31.2	0.312	0.321	0.317	0.151
15.6	0.255	0.268	0.262	0.096
0	0.164	0.167	0.166

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Analgesic Effect of Dexmedetomidine-Nalbuphine Combination vs. Dexmedetomidine Alone in Donkeys Undergoing Field Castration under Total Intravenous Anesthesia IE Helal, HA Al-Abbadi, MA Hashem, HMA Abdelrazek,Animals,2024
Expression of Vitamin D Receptor Gene (VDR) and Associated Cytokine Response in Arabian Horses with Chronic Inflammatory Airway Disease H Haddadi,/,2025
Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells Abdelhamid L.et al,Res Vet Sci,2017

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能参考文献：
1. The results obtained showed that IL-6 expression in adipose tissue biopsies derived from animals with equine metabolic syndrome was enhanced while TNF-alpha levels of both groups were comparable. PMID: 25269712
2. Data indicate that tumor necrosis factor-alpha production is closely related to ovarian steroid actions. PMID: 24642167
3. These results indicate that in equine corpus luteum, cytokines TNF, IFNG and FASL regulate nitric oxide activity, via eNOS expression modulation. PMID: 23941776
4. These data show the presence of cytokines TNF and IFNG, and their receptors, in the equine corpus luteum and indicate their potential involvement in regulation of luteal function. PMID: 22186103
5. These results suggest that basal oxidative stress markers, circulating cytokines and anti-inflammatory neuroendocrine hormones appear to correlate with endurance performance in horses. PMID: 21058993
6. The contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of IL-6, IL-10 and TNF-alpha, in horses with recurrent airway obstruction during exacerbation and in remission is reported. PMID: 18294392
7. The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID: 18584861
显示更多

收起更多
亚细胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
数据库链接：

KEGG: ecb:100033834

STRING: 9796.ENSECAP00000001460

UniGene: Eca.13019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.