Horse Progesterone(PROG) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO马孕激素/孕酮（PROG）酶联免疫检测试剂盒（货号：CSB-E13183Hs），基于竞争法原理实现血清和血浆样本中孕酮的定量分析，检测灵敏度达0.25 ng/mL，线性范围覆盖0.25-100 ng/mL。孕酮是调控雌性动物生殖功能的关键激素，参与发情周期调节、胚胎着床及妊娠维持等生理过程，其水平监测对马匹繁殖管理、妊娠诊断及生殖疾病研究具有重要意义。CUSABIO试剂盒采用高特异性抗体，可精准识别样本中的孕酮分子，适用于科研场景下的马科动物生殖生理研究、妊娠状态追踪、繁殖效率评估及激素代谢分析。实验流程经优化设计，配套标准品及反应体系完备，可在常规实验室条件下快速完成检测，为畜牧业繁殖优化、野生动物保护等领域的激素水平研究提供可靠工具。
缩写：

PROG
种属：

Equus caballus (Horse)
样本类型：

serum, plasma
检测范围：

0.25 ng/mL-100 ng/mL
灵敏度：

0.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-107
1:2	Average %	89
	Range %	83-99
1:4	Average %	95
	Range %	85-103
1:8	Average %	90
	Range %	86-102

回收率：

The recovery of horse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-106
EDTA plasma (n=4)	95	82-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average
100	0.162	0.158	0.160
18.75	0.320	0.332	0.326
3.125	0.542	0.563	0.553
0.875	0.971	0.944	0.958
0.25	1.503	1.485	1.494
0	2.192	2.127	2.160

Evaluation of circulating miRNAs in mares approaching parturition M Kikuchi, H Murase, K Urata, T Ishige, T Tozaki,Domestic Animal Endocrinology,2024

最新研究进展：

PROG是一种激素，可通过作用于孕酮受体来调节生殖系统、乳房和神经系统的功能。近年来，PROG被广泛研究，尤其在抗肿瘤治疗和神经保护方面的应用潜力备受关注。多项研究表明，PROG可以通过多种机制发挥抗肿瘤作用，如调节细胞周期、促进细胞凋亡、抑制血管生成等。同时，PROG也具有保护神经元、促进神经元再生的作用，可以减轻多种神经退行性疾病的症状。这些研究为PROG的临床应用提供了新思路和方向。