Horse Gelsolin(GSN) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

马凝溶胶蛋白(GSN)酶联免疫试剂盒（CSB-EL009965HO）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GSN含量。GSN 是一个靶点。它在细胞中具有重要作用，与细胞的运动、形态维持等相关。研究机制方面，其可能参与调节细胞骨架的动态变化。通过对它的深入研究，有望揭示疾病发生发展机制，为开发新的治疗方法和药物提供方向。试剂盒检测范围为12.5 ng/mL-800 ng/mL，该产品可为马属动物细胞迁移机制、炎症相关疾病模型或肌肉组织再生等基础研究提供关键数据支持，适用于实验室条件下对生物样本中GSN蛋白表达水平的动态监测及比较分析，为探索其分子功能及调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

GSNGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
缩写：

GSN
Uniprot No.：

Q28372
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

6.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GSN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	94-105
1:2000	Average %	89
	Range %	82-94
1:4000	Average %	102
	Range %	98-106
1:8000	Average %	95
	Range %	91-99

回收率：

The recovery of horse GSN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	91	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
基因功能参考文献：
1. Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin PMID: 19666512
亚细胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Villin/gelsolin family
数据库链接：

KEGG: ecb:100034186

UniGene: Eca.2517