Horse Erythropoietin(EPO) ELISA kit

产品详情

产品描述：

CUSABIO马红细胞生成素（EPO）酶联免疫检测试剂盒（货号：CSB-EL007743HO），基于双抗体夹心法（Sandwich ELISA）原理设计，能够定量检测血清、血浆、组织匀浆及细胞裂解液中的EPO蛋白，检测灵敏度范围为0.156 ng/mL至10 ng/mL。EPO是由肾脏分泌的关键调节激素，通过促进红细胞生成维持机体氧平衡，在贫血代偿、缺氧适应及骨髓造血功能调控中具有重要作用。该试剂盒适用于科研领域探索EPO在生理或病理条件下的表达规律，例如研究贫血相关疾病机制、慢性肾病引起的红细胞生成异常、肿瘤微环境对造血功能的干扰，或评估药物及生物活性分子对EPO信号通路的影响。实验方案兼容多种生物样本类型，操作简便且稳定性高，可为体外实验提供精准的定量数据支持，助力于血液学、肿瘤生物学及代谢调控等领域的分子机制研究。
别名：

EPOErythropoietin ELISA Kit
缩写：

EPO
Uniprot No.：

Q867B1
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of horse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	106	101-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
亚细胞定位：

Secreted.
蛋白家族：

EPO/TPO family
数据库链接：

KEGG: ecb:100033849

STRING: 9796.ENSECAP00000008707

UniGene: Eca.12758