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  4. Horse Clusterin(CLU) ELISA kit

Horse Clusterin(CLU) ELISA kit

说明书下载
  • 中文名称：
    马凝聚素(CLU)酶联免疫试剂盒
  • 货号：
    CSB-EL005595HO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    马凝聚素(CLU)酶联免疫试剂盒（CSB-EL005595HO）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CLU含量。CLU（Clusterin）是一种存在于多种细胞组分中的多功能蛋白，最早发现于山羊精液中，参与细胞间黏附。研究显示CLU与神经退行性疾病、免疫疾病、衰老和癌症等多种病理状态相关。CLU具有不同的蛋白产物，如分泌型（sCLU）和核型（nCLU），在细胞定位和生物学功能上有所不同。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，本产品适用于科研领域对CLU蛋白表达水平的精准分析，为研究CLU在心血管疾病、神经退行性病变及肿瘤微环境中的分子机制提供了可靠工具，也可用于评估实验动物模型或体外培养体系中CLU的干预调控效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CLU ELISA Kit; Clusterin [Cleaved into: Clusterin beta chain; Clusterin alpha chain] ELISA Kit
  • 缩写：
    CLU
  • Uniprot No.：
  • 种属：
    Equus caballus (Horse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of horse CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 101
    Range % 95-106
    1:2000 Average % 90
    Range % 85-94
    1:4000 Average % 98
    Range % 92-102
    1:8000 Average % 100
    Range % 94-104
  • 回收率：
    The recovery of horse CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 86-96
    EDTA plasma (n=4) 102 95-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    1000 0.171 0.167 0.169
    500 0.277 0.264 0.271
    250 0.498 0.473 0.486
    125 0.811 0.819 0.815
    62.5 1.349 1.330 1.340
    31.25 1.906 1.878 1.892
    15.6 2.412 2.335 2.374
    0 2.950 2.876 2.913
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

CLU Proteins

靶点详情

  • 功能：
    Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Following stress, promotes apoptosis. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury.
  • 基因功能参考文献：
    1. The secretion of the potential zonal marker clusterin by zonal articular chondrocytes in osteoarthritic and healthy articular cartilage, was characterized. PMID: 19814590
  • 亚细胞定位：
    Secreted. Nucleus. Cytoplasm. Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Mitochondrion. Mitochondrion membrane. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, chromaffin granule.
  • 蛋白家族：
    Clusterin family
  • 数据库链接：

    KEGG: ecb:100034172

    STRING: 9796.ENSECAP00000005450

    UniGene: Eca.18000

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |