Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO仓鼠脂联素（ADIPOQ）酶联免疫检测试剂盒（货号：CSB-EL001366HA），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的ADIPOQ蛋白浓度，检测灵敏度达0.469 ng/mL，线性范围为0.469-30 ng/mL。ADIPOQ是由脂肪细胞分泌的代谢调节蛋白，通过激活AMPK和PPAR-α信号通路参与胰岛素敏感性调节、脂肪酸氧化及抗炎反应，在肥胖、糖尿病等代谢性疾病研究中具有重要价值。试剂盒通过预包被特异性抗体捕获目标蛋白，结合生物素标记的二抗形成复合物，经链霉亲和素-HRP催化显色反应实现定量分析，操作流程约3.5小时完成。该产品适用于科研实验中评估仓鼠模型在代谢疾病干预、脂肪组织功能研究、运动生理学或药物开发过程中ADIPOQ表达水平的变化，为探索脂联素在能量代谢调控机制提供可靠工具，试剂盒包含标准品、检测抗体及必要反应组分，需按说明书进行样本前处理及-20℃保存。
缩写：

ADIPOQ
Uniprot No.：

C9DFG2
种属：

Mesocricetus auratus (Golden hamster)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.469 ng/mL-30 ng/mL
灵敏度：

0.117 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	82-90
1:2000	Average %	93
	Range %	90-96
1:4000	Average %	89
	Range %	84-93
1:8000	Average %	94
	Range %	90-98

回收率：

The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.