Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

产品详情

引用文献

产品详情

引用文献

产品评价

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

仓鼠假定磷脂酶B样蛋白2(PLBD2)酶联免疫试剂盒
货号：

CSB-EL018125HA
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献

产品描述：

CUSABIO仓鼠假定磷脂酶B样蛋白2（PLBD2）酶联免疫检测试剂盒（货号：CSB-EL018125HA），基于双抗体夹心法原理设计，适用于定量检测血清、血浆、组织匀浆及细胞裂解液等多种样本中的PLBD2蛋白水平。PLBD2作为磷脂酶B家族推定成员，可能参与脂质代谢或膜结构调控过程，其表达特征与细胞生理活动密切相关。该试剂盒检测灵敏度为12.5 pg/mL，线性检测范围覆盖12.5-800 pg/mL，通过预包被高特异性捕获抗体与标记检测抗体的协同作用，实现对目标蛋白的精准定量分析。科研人员可将其应用于基础研究领域，例如探索PLBD2在脂质代谢途径中的功能机制、评估特定疾病模型中该蛋白的表达动态变化，或用于细胞信号转导相关实验的分子标记物检测。试剂盒严格匹配仓鼠物种特异性，配套提供标准品、检测抗体及显色系统，实验全程无需额外制备抗体，适用于分子生物学实验室常规蛋白定量研究需求。
别名：

P76, LAMA-like protein 2|PLB homolog 2|lamina ancestor homolog 2|mannose-6-phosphate protein associated protein p76|phospholipase B-like 2 32 kDa form|phospholipase B-like 2 45 kDa form
缩写：

PLBD2
种属：

Mesocricetus auratus (Golden hamster)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster PLBD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-105
1:2	Average %	92
	Range %	86-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	91-100

回收率：

The recovery of hamster PLBD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	89-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.349	2.367	2.358	2.227
400	1.975	1.924	1.950	1.819
200	1.408	1.466	1.437	1.306
100	0.912	0.857	0.885	0.754
50	0.555	0.521	0.538	0.407
25	0.363	0.374	0.369	0.238
12.5	0.248	0.259	0.254	0.123
0	0.133	0.129	0.131

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Method of Characterization of Visible and/or Sub-Visible Particles in Biologics X Xu,freepatentsonline,2020
Compositions and methods for detecting and quantifying host cell protein in cell lines and recombinant polypeptide products Feny Gunawan, et al,/,2018

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.