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  4. Hamster Interleukin-4(IL4) ELISA kit

Hamster Interleukin-4(IL4) ELISA kit

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  • 中文名称：
    仓鼠白介素4(IL4)酶联免疫试剂盒
  • 货号：
    CSB-EL011659HA
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO仓鼠白介素4（IL-4）酶联免疫试剂盒（货号：CSB-EL011659HA），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中IL-4蛋白的科研分析。IL-4是由Th2细胞分泌的重要细胞因子，在免疫调节中发挥核心作用，参与B细胞活化、抗体类别转换及过敏反应等生理病理过程。试剂盒检测范围宽达9.37-600 pg/mL，最低检测灵敏度为9.37 pg/mL，采用预包被特异性抗体的96孔板结构，配合HRP酶标记检测抗体及TMB显色系统，可精准捕捉样本中IL-4的含量变化。实验流程包含标准品梯度稀释、样本孵育、洗涤及显色步骤，配套提供即用型冻干标准品和实验所需试剂，操作便捷且重复性强。该试剂盒适用于仓鼠免疫机制研究、炎症性疾病模型评估、疫苗或药物开发中的免疫应答分析等科研场景，为探究Th2型免疫反应、过敏性疾病机制或感染病理过程提供可靠工具，实验全程需避免反复冻融样本以保证检测准确性。
  • 别名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 缩写：
    IL4
  • Uniprot No.：
  • 种属：
    Mesocricetus auratus (Golden hamster)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    9.37 pg/mL-600 pg/mL
  • 灵敏度：
    2.34 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 90-101
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 100
    Range % 98-104
    1:8 Average % 94
    Range % 87-97
  • 回收率：
    The recovery of hamster IL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 93-99
    EDTA plasma (n=4) 91 87-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    600 1.820 1.864 1.842 1.628
    300 1.357 1.289 1.323 1.109
    150 0.930 0.963 0.947 0.733
    75 0.706 0.739 0.723 0.509
    37.5 0.503 0.524 0.514 0.300
    18.75 0.392 0.384 0.388 0.174
    9.37 0.304 0.288 0.296 0.082
    0 0.216 0.212 0.214 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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IL4 Proteins

靶点详情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |