Hamster Interleukin-10(IL10) ELISA kit

产品详情

产品描述：

CUSABIO仓鼠白介素10（IL10）酶联免疫检测试剂盒（货号：CSB-EL011580HA），采用夹心法原理实现定量分析，适用于科研领域对血清、血浆及组织匀浆样本中IL10蛋白含量的精准检测。IL10作为重要的抗炎细胞因子，在免疫调节和炎症反应中发挥关键作用，其水平变化常被用于评估感染性疾病、自身免疫病等病理模型的免疫状态。该试剂盒检测范围覆盖6.25 pg/mL至400 pg/mL的宽线性区间，可灵敏捕捉样本中微量IL10的动态变化，尤其适用于仓鼠炎症模型研究、疫苗免疫效果评价或药物干预实验中的生物标志物分析。实验操作中，样本无需复杂前处理，血清和血浆可直接用于检测，而组织匀浆则能有效反映局部组织微环境的IL10表达特征。试剂盒包含预包被特异性抗体的酶标板、标准品及检测所需全套试剂组分，提供稳定可靠的检测重复性，满足体外实验中细胞因子定量研究的多样化需求。
别名：

CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
缩写：

IL10
种属：

Mesocricetus auratus (Golden hamster)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	91-105
1:2	Average %	106
	Range %	97-110
1:4	Average %	95
	Range %	87-99
1:8	Average %	93
	Range %	84-97

回收率：

The recovery of hamster IL10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-97
EDTA plasma (n=4)	95	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.