Hamster C-reactive protein(CRP) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO仓鼠C反应蛋白（CRP）酶联免疫检测试剂盒（货号：CSB-EL005991HA），采用双抗体夹心法定量分析血清、血浆及组织匀浆样本中的CRP水平。C反应蛋白是一种由肝脏合成的急性时相反应蛋白，在炎症、感染或组织损伤等生理病理过程中显著升高，常用于评估机体炎症反应强度及疾病进程。本试剂盒检测灵敏度高，线性范围为12.5-800 ng/mL，可精准覆盖仓鼠样本中CRP的基础浓度及动态变化范围。实验操作采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立标准曲线，配合酶标仪在450 nm波长下完成吸光度读数，实现目标蛋白的定量分析。适用于仓鼠模型中的炎症机制研究、药物干预效果评价或疾病相关生物标志物探索等科研场景，为实验动物模型的炎症状态监测提供可靠工具。试剂盒包含完整实验所需组分（标准品、检测抗体、显色底物等），支持手动或自动化操作流程。
别名：

CRP ELISA kit; PTX1C-reactive protein ELISA kit
缩写：

CRP
Uniprot No.：

P49262
种属：

Mesocricetus auratus (Golden hamster)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	85-96
1:10	Average %	87
	Range %	82-92
1:20	Average %	96
	Range %	89-103
1:40	Average %	98
	Range %	94-104

回收率：

The recovery of hamster CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-93
EDTA plasma (n=4)	96	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
亚细胞定位：

Secreted.
蛋白家族：

Pentraxin family
组织特异性：

Found in plasma.