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中文名称：豚鼠单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒
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货号：CSB-E13559Gp
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：CUSABIO豚鼠单核细胞趋化蛋白1（MCP-1/CCL2/MCAF）酶联免疫检测试剂盒（货号：CSB-E13559Gp），基于双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本分析，检测灵敏度达23.5 pg/mL，线性范围为23.5-1500 pg/mL。MCP-1作为CCL2趋化因子家族成员，在炎症反应和免疫调控中发挥重要作用，其表达水平与感染性疾病、自身免疫病理过程密切相关。该试剂盒通过特异性识别豚鼠MCP-1分子，为科研人员提供精准的检测工具，可应用于炎症机制研究、免疫调节实验、疾病模型建立等基础研究领域。例如在豚鼠感染模型或自身免疫性疾病模型中，可通过监测血清或组织样本中MCP-1的动态变化，揭示疾病进程中趋化因子的调控规律。试剂盒操作流程符合标准ELISA技术规范，配备优化后的预混试剂，确保实验高效稳定，为豚鼠相关科研实验提供可靠的数据支持。
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别名：CCL2 ELISA Kit; MCP1 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Guinea Sus scrofa (Pig)
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样本类型：serum, plasma, tissue homogenates
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检测范围：23.5 pg/mL-1500 pg/mL
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灵敏度：5.8 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 102 Range % 97-107 1:4 Average % 91 Range % 85-97 1:8 Average % 97 Range % 91-103 -
回收率：
The recovery of guinea pig MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 93 87-98 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1500 1.857 1.902 1.880 1.680 750 1.345 1.403 1.374 1.174 375 0.981 0.954 0.968 0.768 187 0.748 0.700 0.724 0.524 94 0.511 0.532 0.522 0.322 47 0.416 0.426 0.421 0.221 23.5 0.289 0.281 0.285 0.085 0 0.201 0.199 0.200 -
数据处理：
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货期：3-5 working days
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靶点详情
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功能：Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
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亚细胞定位：Secreted.
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蛋白家族：Intercrine beta (chemokine CC) family
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数据库链接：
STRING: 10141.ENSCPOP00000012126
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