Guinea pig interleukin 5,IL-5 ELISA Kit

Guinea pig interleukin 5,IL-5 ELISA Kit

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中文名称：

豚鼠白介素5(IL-5)酶联免疫试剂盒
货号：

CSB-E12064Gp
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO豚鼠白介素5（IL-5）酶联免疫检测试剂盒（货号：CSB-E12064Gp），采用双抗体夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中IL-5的表达水平。IL-5作为Th2细胞分泌的关键细胞因子，在豚鼠模型中主要参与调控嗜酸性粒细胞分化与活化，与过敏性炎症反应和抗寄生虫免疫研究密切相关。试剂盒检测范围覆盖31.25 pg/mL至2000 pg/mL的宽线性区间，可精准检测低浓度样本，标准品为冻干粉形式提供，需按说明书进行梯度稀释。实验操作需使用酶标仪在450 nm波长下读数，建议样本离心后取上清检测以避免颗粒物干扰。该试剂盒适用于豚鼠疾病模型的免疫机制研究，例如哮喘、寄生虫感染等实验中的炎症因子动态监测，或用于评估免疫调节药物对IL-5通路的影响。组织匀浆样本可用于分析肺、骨髓等靶器官的局部IL-5表达水平，为科研人员提供稳定可靠的定量数据支持。
别名：

IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
缩写：

IL5
Uniprot No.：

O08987
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	94
	Range %	89-99
1:4	Average %	103
	Range %	98-108
1:8	Average %	100
	Range %	91-105

回收率：

The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-97
EDTA plasma (n=4)	92	84-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.516	2.489	2.503	2.391
1000	1.384	1.356	1.370	1.258
500	0.733	0.698	0.716	0.604
250	0.384	0.375	0.380	0.268
125	0.253	0.234	0.244	0.132
62.5	0.187	0.181	0.184	0.072
31.25	0.142	0.133	0.138	0.026
0	0.113	0.111	0.112

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Altered metabolites in guinea pigs with allergic asthma after acupoint sticking therapy: new insights from a metabolomics approach Shi-jian Xiang.et al,Phytomedicine,2018
Therapeutic effects of naringin in a guinea pig model of ovalbumin-induced cough-variant asthma Jiao HY. et al,Pulm Pharmacol Ther,2015

功能：

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
亚细胞定位：

Secreted.
蛋白家族：

IL-5 family
数据库链接：

STRING: 10141.ENSCPOP00000004355

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.