Guinea pig interleukin 4,IL-4 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于豚鼠白介素4（IL-4）检测的酶联免疫试剂盒（货号CSB-E10126Gu），采用双抗体夹心法原理实现精确定量。IL-4是由Th2细胞分泌的关键细胞因子，在免疫调节中发挥重要作用，包括促进B细胞增殖分化、IgE抗体产生及过敏反应调控等生物学过程。试剂盒可检测血清、血浆及组织匀浆样本，检测范围宽达3.12至200皮克/毫升，灵敏度达最低检测限3.12 pg/mL，标准品采用梯度预包被设计，用户仅需进行样本处理和显色读数即可获得定量结果。该试剂盒适用于豚鼠模型的免疫机制研究，特别是在过敏性疾病、寄生虫感染、哮喘模型等Th2型免疫反应相关科研领域，为评估实验动物体内IL-4动态变化提供可靠工具。配套提供标准品、预包被板及优化缓冲液体系，操作流程经过严格验证，实验全程约需4.5小时，兼容常规酶标仪在450nm波长下的吸光度读取。本产品专为科研实验设计，适用于体外检测，不涉及活体诊断用途。
缩写：

IL-4
Uniprot No.：

G0WM66
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-105
1:2	Average %	96
	Range %	92-102
1:4	Average %	103
	Range %	97-107
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of guinea pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.