Guinea pig interleukin 2,IL-2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于豚鼠样本检测的白介素2（IL-2）酶联免疫试剂盒（货号CSB-E12914Gp），采用双抗体夹心法原理实现31.25-2000 pg/mL范围的精确定量分析。IL-2作为介导T淋巴细胞增殖与活化的核心细胞因子，在免疫应答调控、自身免疫性疾病及感染机制研究中具有重要价值。该试剂盒支持血清、血浆及组织匀浆三类样本检测，预包被特异性抗体可稳定结合目标蛋白，配合优化的反应体系确保检测灵敏度达31.25 pg/mL，试剂组分包含标准品、检测抗体及显色液，建议保存于-20℃并注意避光。适用于科研领域探究豚鼠感染模型中的免疫激活状态、评估疫苗或免疫调节剂的干预效果，以及炎症性疾病机制研究。实验全程约需4.5小时，建议新鲜样本2小时内完成预处理，冻存样本避免反复冻融以保障检测准确性。本产品通过种属特异性抗体设计有效避免交叉反应，为豚鼠免疫学研究提供可靠工具。
缩写：

IL-2
Uniprot No.：

H0VBY4
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-108
1:2	Average %	94
	Range %	91-98
1:4	Average %	103
	Range %	97-108
1:8	Average %	88
	Range %	80-96

回收率：

The recovery of guinea pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-100
EDTA plasma (n=4)	104	96-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.