Guinea pig interleukin 13,IL-13 ELISA Kit

产品详情

产品描述：

CUSABIO豚鼠白介素13（IL-13）酶联免疫检测试剂盒（货号：CSB-E12916Gp），专为科研领域设计，用于定量分析血清、血浆及组织匀浆样本中IL-13蛋白的浓度水平。IL-13作为Th2型细胞分泌的关键细胞因子，在过敏反应、哮喘及寄生虫免疫应答中发挥重要调节作用，豚鼠模型因其呼吸道生理特性常被用于相关机制研究。试剂盒基于双抗体夹心法原理，通过高特异性的捕获抗体和检测抗体实现精准定量，检测灵敏度达7.5 pg/mL，线性检测范围覆盖7.5-300 pg/mL，可有效解析不同实验条件下IL-13的细微浓度变化。该试剂盒适用于豚鼠感染模型、呼吸道炎症研究、免疫调节药物开发等科研场景，能够稳定检测包括肺组织匀浆在内的多种生物样本，为探索IL-13在免疫调控网络中的作用及评估实验性治疗方案的生物学效应提供可靠工具。配套试剂经严格质控，包含预包被酶标板、标准品、显色液等完整组分，操作流程标准化，实验重复性良好。
缩写：

IL-13
Uniprot No.：

H0W834
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.5 pg/mL-300 pg/mL
灵敏度：

3.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-92
1:2	Average %	95
	Range %	85-105
1:4	Average %	93
	Range %	86-102
1:8	Average %	97
	Range %	92-110

回收率：

The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-103
EDTA plasma (n=4)	93	86-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.