Guinea pig interleukin 10,IL-10 ELISA Kit

Guinea pig interleukin 10,IL-10 ELISA Kit

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中文名称：

豚鼠白介素10(IL-10)酶联免疫试剂盒
货号：

CSB-E12915Gp
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专用于豚鼠样本的白介素10（IL-10）定量检测试剂盒，采用双抗体夹心酶联免疫吸附法（Sandwich ELISA），能够精准检测血清、血浆及组织匀浆中31.25-2000 pg/mL浓度范围的IL-10蛋白。IL-10作为关键的抗炎细胞因子，在调节免疫反应、抑制炎症介质释放中发挥核心作用，其动态监测对豚鼠模型中炎症性疾病机制、免疫调控研究及药物干预效果评估具有重要意义。试剂盒包含预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配合标准品与显色底物完成定量分析。实验全程可在4-6小时内完成，适用于科研场景下豚鼠感染性疾病模型、过敏反应研究、免疫调节剂开发等领域的IL-10表达水平分析，尤其适合评估实验药物或生物制剂对免疫通路的影响。试剂盒经严格的交叉反应验证，确保对豚鼠IL-10的高度特异性识别，为研究者提供稳定可靠的实验工具。
别名：

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：

IL10
Uniprot No.：

Q9Z1Y5
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	96
	Range %	91-103
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	91-100

回收率：

The recovery of guinea pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-101
EDTA plasma (n=4)	97	91-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.894	2.903	2.899	2.780
1000	2.107	2.115	2.111	1.992
500	1.233	1.241	1.237	1.118
250	0.756	0.767	0.762	0.643
125	0.443	0.451	0.447	0.328
62.5	0.289	0.299	0.294	0.175
31.25	0.184	0.197	0.191	0.072
0	0.118	0.120	0.119

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Isoleucine attenuates infection induced by E. coli challenge through modulation of intestinal endogenous antimicrobial peptide expression and inhibition of the increase of plasma endotoxin and IL-6 in weaned pigs Man Ren,Food & Function,2019
Dietary xylo-oligosaccharide improves intestinal function in weaned piglets Jie Yin, et al,Food & Function,2019

功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
亚细胞定位：

Secreted.
蛋白家族：

IL-10 family

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.