Guinea pig insulin (INS) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

豚鼠胰岛素（INS）酶联免疫试剂盒（货号：CSB-EL011742GU）采用夹心法原理设计，可定量检测血清、血浆及组织匀浆样本中的胰岛素含量，检测灵敏度为12 μIU/mL，线性范围为12-210 μIU/mL，适用于豚鼠模型中胰岛素相关科研分析。胰岛素作为调节糖代谢的核心激素，其浓度变化可反映机体能量代谢状态，在糖尿病机制研究、代谢性疾病模型构建及药物干预效果评价等领域具有重要应用价值。本试剂盒通过双抗体夹心法特异性捕获样本中的胰岛素分子，结合酶标反应显色，实现稳定定量检测，兼容多种生物样本类型，操作流程标准化，可为胰腺功能研究、胰岛素分泌调控实验提供精准数据支持。该产品严格适用于实验室科研用途，不涉及任何临床或诊断用途，实验结果需结合其他生物学指标综合分析。
别名：

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
缩写：

INS
Uniprot No.：

P01329
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12 μIU/mL-210 μIU/mL
灵敏度：

7.5 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-97
1:2	Average %	96
	Range %	90-106
1:4	Average %	95
	Range %	86-104
1:8	Average %	90
	Range %	87-94

回收率：

The recovery of guinea pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-101
EDTA plasma (n=4)	95	89-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
亚细胞定位：

Secreted.
蛋白家族：

Insulin family
数据库链接：

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