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  4. Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

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  • 中文名称：
    豚鼠肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
  • 货号：
    CSB-E06772Gu
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    豚鼠肿瘤坏死因子α(TNF-α)酶联免疫试剂盒（货号：CSB-E06772Gu）专为科研领域设计，用于定量检测豚鼠血清、血浆及组织匀浆样本中的TNF-α水平。TNF-α是一种关键的促炎细胞因子，在宿主防御、炎症反应及免疫调节中发挥重要作用，其表达水平的变化常用于评估感染性疾病、自身免疫反应或肿瘤研究中的免疫状态。CUSABIO试剂盒采用双抗体夹心法原理，检测灵敏度达3.12 pg/mL，线性检测范围为3.12-200 pg/mL，可精准覆盖生物样本中TNF-α的浓度梯度。试剂盒兼容多种样本类型，能够满足不同实验设计的需求，例如通过检测组织匀浆中的TNF-α水平分析局部炎症反应，或利用血液样本评估系统性免疫激活状态。该产品适用于基础科研中炎症机制研究、药物干预效果评价以及豚鼠模型的免疫相关疾病实验，操作流程标准化，实验结果稳定可靠，为研究者提供高效便捷的检测工具。
  • 别名：
    TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
  • 缩写：
    TNF
  • Uniprot No.：
  • 种属：
    Guinea Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %90-100
    1:2Average %94
    Range %89-98
    1:4Average %92
    Range %87-96
    1:8Average %93
    Range %89-100
  • 回收率：
    The recovery of guinea pig TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-97
    EDTA plasma (n=4)9792-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.350 2.376 2.363 2.200
    1001.884 1.902 1.893 1.730
    501.368 1.379 1.374 1.211
    250.961 0.973 0.967 0.804
    12.50.664 0.681 0.673 0.510
    6.250.475 0.486 0.481 0.318
    3.120.305 0.309 0.307 0.144
    00.162 0.164 0.163 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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产品评价

平均分:
5.0分 - 1 个评价

样品类型：组织匀浆液 肺组织匀浆上清

样品信息：其他 SPF级豚鼠

稀释比：没有稀释

产品评价： 我通过使用CSB-E06772Gu检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师

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TNF Proteins

靶点详情

  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：

    STRING: 10141.ENSCPOP00000002129

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