Guinea pig Interleukin 8,IL-8 ELISA Kit

Guinea pig Interleukin 8,IL-8 ELISA Kit

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中文名称：

豚鼠白介素8(IL-8)酶联免疫试剂盒
货号：

CSB-E13096Gp
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO针对豚鼠白介素8（IL-8/CXCL8）检测设计的双抗体夹心法酶联免疫试剂盒（货号：CSB-E13096Gp），适用于血清、血浆及组织匀浆样本的定量分析。IL-8作为趋化因子家族重要成员，在豚鼠炎症反应中通过介导中性粒细胞趋化作用参与宿主防御机制，其水平变化常作为炎症相关研究的生物标志物。试剂盒采用高特异性配对抗体，可在31.25-2000 pg/mL范围内实现精准定量，最低检测限达12.5 pg/mL，标准曲线相关系数R²＞0.99，支持96孔板规格的批量样本检测。样本兼容性强，血清或血浆样本可直接检测，组织匀浆仅需常规离心处理即可上样，操作全程可在3.5小时内完成。该试剂盒广泛应用于豚鼠模型的感染性疾病机制研究、免疫调节实验及药物开发中的炎症因子动态监测，特别适用于呼吸道感染、皮肤炎症或肠道疾病等动物模型的病理分析，为科研人员提供稳定可靠的IL-8浓度检测方案。
别名：

CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant protein 1 ELISA Kit; NAP-1 ELISA Kit
缩写：

CXCL8
Uniprot No.：

P49113
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	95
	Range %	85-100
1:4	Average %	95
	Range %	88-102
1:8	Average %	97
	Range %	90-104

回收率：

The recovery of guinea pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-102
EDTA plasma (n=4)	94	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.253	2.245	2.249	2.134
1000	1.631	1.696	1.664	1.549
500	1.111	1.135	1.123	1.008
250	0.717	0.752	0.735	0.620
125	0.434	0.423	0.429	0.314
62.5	0.343	0.354	0.349	0.234
31.25	0.228	0.241	0.235	0.120
0	0.116	0.113	0.115

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Suhuang Antitussive Capsule Ameliorates Corticosteroid Insensitivity in Cough Variant Asthma Guinea Pigs by Inhibiting p38 MAPK Signal Pathway T Lyu,Evidence-based complementary and alternative medicine,2023
Follicular fluid exosomes inhibit BDNF expression and promote the secretion of chemokines in granulosa cells by delivering miR-10b-5p C Yuan,Theriogenology,2023
Up-regulation of microRNA-335-5p Reduces Inflammation via Negative Regulation of the TPX2-mediated AKT/GSK3β Signaling Pathway in a Chronic Rhinosinusitis Mouse Model X Gu,Cellular Signalling,2020
Effect of folate supplementation on immunological and autophagy markers in experimental nonalcoholic fatty liver disease T Minato,European Cytokine Network volume,2019
Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
Maternal dietary resveratrol alleviates weaning-associated diarrhea and intestinal inflammation in pig offspring by changing intestinal gene expression and microbiota Qingwei Meng, et al,Food & Function,2019
Mycoplasma hyopneumoniae Mhp597 is a Cytotoxicity, Inflammation and Immunosuppression Associated Nuclease PengLi,Veterinary Microbiology,2019
Arginase inhibition prevents inflammation and remodeling in a guinea pig model of COPD Pera T et al,J Pharmacol Exp Ther,2014

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5.0分 - 1 个评价

样品类型：组织匀浆液肺组织匀浆上清

样品信息：其他 SPF级豚鼠

稀释比：没有稀释

产品评价：我通过使用CSB-E13096Gp检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师

功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
亚细胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
数据库链接：

STRING: 10141.ENSCPOP00000009984

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.