Guinea pig Interleukin-1 beta(IL1B) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

豚鼠白介素1β（IL1B）酶联免疫试剂盒（货号：CSB-EL011614GU）基于双抗体夹心法原理开发，可对血清、血浆及组织匀浆样本中的IL1B蛋白进行定量检测，灵敏度达15.6 pg/mL，检测范围为15.6-1000 pg/mL。IL1β作为关键的促炎细胞因子，在炎症反应、免疫调节及细胞增殖分化中发挥重要作用，其表达水平与感染性疾病、自身免疫疾病及组织损伤修复等过程密切相关。本试剂盒通过高特异性抗体对实现靶标捕获与检测，适用于豚鼠模型的基础科研领域，如炎症机制研究、免疫相关疾病动物模型分析、药物或化合物抗炎效果评估等场景。实验操作兼容常规ELISA流程，能够稳定检测生物样本中IL1β的动态变化，为探索豚鼠在病原体感染、创伤修复或免疫干预实验中IL1β的调控机制提供可靠工具，满足实验室对炎症标志物的精准定量需求。
别名：

IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：

IL1B
Uniprot No.：

Q9WVG1
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	97
	Range %	85-100
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of guinea pig IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	92	88-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
亚细胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
数据库链接：

STRING: 10141.ENSCPOP00000001784