Guinea pig Interferon γ,IFN-γ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

豚鼠γ干扰素(IFN-γ)酶联免疫试剂盒（CSB-E06763Gu）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFN-γ含量。IFN-γ即γ-干扰素，是具有多种生物学活性的细胞因子。背景上，它在免疫调节等机体生理功能中扮演重要角色。研究机制方面，其可激活巨噬细胞、增强NK细胞活性等，在抗感染、抗肿瘤及免疫相关疾病研究中受广泛关注。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，尤其适用于豚鼠模型的感染性疾病机制探索和疫苗开发等科研领域；适用于评估免疫激活状态或炎症反应强度；该产品广泛应用于基础医学、兽医学研究中宿主免疫应答分析、病原体感染模型建立以及生物制剂效果评价等非诊断性科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IFN-gamma，interferon gamma
缩写：

IFN-γ
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.7 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	97
	Range %	92-103
1:4	Average %	93
	Range %	88-108
1:8	Average %	101
	Range %	96-105

回收率：

The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	90	86-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.