Grouper Vitellogenin,VTG ELISA Kit

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中文名称：

石斑鱼卵黄蛋白原(VTG)酶联免疫试剂盒
货号：

CSB-E14116Fh
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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引用文献

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO石斑鱼卵黄蛋白原（VTG）定量检测试剂盒（货号：CSB-E14116Fh），采用竞争法酶联免疫吸附原理开发，专为科研领域设计。卵黄蛋白原是鱼类卵黄形成的关键前体蛋白，其表达水平与生殖周期密切相关，可作为研究鱼类生殖生理、环境内分泌干扰物效应的重要生物标志物。试剂盒可检测血清、血浆及组织匀浆样本，灵敏度覆盖50 ng/mL至2000 ng/mL的线性范围，满足不同浓度梯度的定量分析需求。该试剂盒通过优化的抗体-抗原竞争反应体系实现精准检测，适用于水产养殖中鱼类繁殖力评估、环境污染对生殖系统影响的机制研究，以及水生态毒理学领域的生物监测实验。操作流程包含标准品梯度稀释、样本预处理及酶标板孵育步骤，配套提供完整试剂与操作指南。本产品仅用于科学研究，不涉及任何临床或诊断用途，为海洋生物生殖发育研究及生态毒理评估提供可靠工具。
别名：

vtg1 ELISA Kit; Vitellogenin ELISA Kit; VTG) [Cleaved into: Lipovitellin I ELISA Kit; LVI); Phosvitin ELISA Kit; PV); Lipovitellin II ELISA Kit; LVII)] ELISA Kit
缩写：

VTG
Uniprot No.：

Q5IGP8
种属：

Grouper
样本类型：

serum, plasma, tissue homogenates
检测范围：

50 ng/mL-2000 ng/mL
灵敏度：

50 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-97
1:2	Average %	97
	Range %	91-102
1:4	Average %	95
	Range %	88-103
1:8	Average %	96
	Range %	91-101

回收率：

The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	95	91-101